摘要
目的测定硫磺熏前后黄芪中黄酮类和皂苷类成分的变化,确定硫磺熏对黄芪药材质量的影响,为评价熏硫加工对黄芪质量的影响提供依据。方法对收集的黄芪进行熏硫加工,采用HPLC-DAD法测定了毛蕊异黄酮苷、芒柄花苷、毛蕊异黄酮、芒柄花素4种黄酮成分,UPLC-ELSD法测定了黄芪皂苷I、黄芪皂苷II、黄芪皂苷III、黄芪甲苷4种皂苷成分。结果硫磺熏过的黄芪药材中毛蕊异黄酮苷、芒柄花苷含量降低,毛蕊异黄酮、芒柄花素、黄芪皂苷I、黄芪皂苷III、黄芪甲苷的含量变化不明显。结论黄芪熏硫加工后,黄酮苷成分有所降低,其余成分变化不明显。
Objective To analyze the contents of flavonoids and saponins in Astragali Radix before and after sulfur-fumigation, so as to provide reliable methods for scientifically evaluating and effectively controlling the quality of Astragali Radix. Methods After sulfur-fumigation, the contents of calycosin glucoside, ononin, calycosin, and formononetin were analyzed by HPLC-DAD, and astragaloside Ⅰ, astragaloside Ⅱ, astragaloside Ⅲ, and astragaloside IV by UPLC-ELSD. Results The contents of calycosin glucoside and ononin decreased, while the contents of calycosin, formononetin, astragaloside Ⅰ, astragaloside Ⅲ, and astragaloside IV were essentially the same. Conclusion After sulfur-fumigation, flavonoid glycoside components are reduced, and the remainder have no obvious change.
出处
《现代药物与临床》
CAS
2014年第5期489-493,共5页
Drugs & Clinic
基金
国家自然科学基金资助项目(81241137)
山西省留学人员科研资助项目(2010-53)
关键词
黄芪
硫磺熏蒸
黄酮
皂苷
变化
Astragali Radix
sulfur-fumigation
flavonoids
saponins
change
