摘要
为了建立从辣椒中提取并通过重结晶获得高纯度辣椒总碱的方法,以花溪朝天椒干粉为原料,采用单因素试验考察了有机溶剂提取法中乙醇浓度和固液比等对提取率的影响,摸索了后续纯化过程中碱溶解-乙酯萃取辣椒碱的最适pH和辣椒碱的结晶条件。结果表明:用95%乙醇、1:10(m/V)的固液比、80℃回流2 h,可达到3.7 mg/g(辣椒总碱/辣椒粉)的提取率;在pH 9.0时用乙酸乙酯萃取辣椒碱的NaOH皂化液可获得较高的辣椒碱纯度和回收率;在实验条件下,该萃取物在甲醇、乙醇、丙酮、乙酸乙酯、氯仿及石油醚等溶剂中均未结晶,而在乙醚中于-20℃重结晶3次得到纯度为96.4%(HPLC法测定)的辣椒总碱,达到美国药典24版(USP24)中的相关要求。
In order to get high pure eapsaicinoids from chilli powder, ethanol was chosen as the extraction solvent and the extraction conditions optimized. The ethanol extract was saponified with 10% NaOH and the optimal pH for extracting eapsaicinoids with ethyl acetate from NaOH solution was screened for. Also, several organic solvents were tried for successful crystallization of capsaicinoids. The results showed that the highest extraction yield was reached when 95% ethanol was used at a solid-liquid ratio of 1：10 （m/V）. A relatively high eapsaieinoids recovery was gained when extracted the saponification solution with ethyl acetate at pH 9.0. Under the experimental conditions, eapsaieinoids were not readily crystallize in methanol, ethanol, acetone, ethyl acetate, chloroform and petroleum ether etc., whereas capsicinoids with purity of 96.4% was obtained after recrystallization in diethyl ether at -20℃ for three times.
出处
《中国农学通报》
CSCD
2014年第12期295-299,共5页
Chinese Agricultural Science Bulletin
基金
贵州省科技计划"贵州省植物生理与发育调控重点实验室建设"(黔科合计Z字[2011]4005号)
教育部长江学者和创新团队发展计划"喀斯特山地生物多样性保护与可持续利用"(PCSIRT1227)
贵州省科技厅农业攻关项目"辣椒抗性资源的筛选鉴定及利用研究"(黔科合NY字[2011]3029号)
贵州省科技厅国际合作项目"辣椒资源的引进
收集及性状研究"(黔科合外G字[2011]7027号)
贵州师范大学学生重点科研项目"辣椒碱类物质提取纯化技术研究"
关键词
辣椒总碱
提取
重结晶
HPLC
capsaicinoids
extraction
recrystallization
HPLC
