摘要
在抗坏血酸／半胱氨酸Maillard体系中，探讨了简单、快速、准确测定抗坏血酸含量的方法。用偏磷酸溶液作为提取剂，比较了紫外分光光度法和高效液相色谱法（HPLC）测定Maillard体系中抗坏血酸的含量。结果表明：紫外分光光度法和HPLC标准曲线线性关系良好，线性范围分别为0～14μg／mL和0-100μg／mL，两种方法相关系数R均达到0．999，平均回收率分别为111％和99．7％。使用两种方法测定三组平行样时，结果相近，误差较小。说明Maillard体系中紫外分光光度法测定抗坏血酸含量时干扰较小，且相比之下其更为简单快捷，适合Maillard体系中的抗坏血酸含量的测定。
The paper explored a simple, rapid and accurate method for the determination of ascorbic acid （Vc） in Maillard reaction from Vc-Cys model system. UV spectrophotometry and HPLC methods were compared using the metaphosphoric acid as the suitable extraction solution. The results showed that both UV spectrophotometry and HPLC methods had good linear correlations,over the measurement range of 0-14μg/mL and 0-100μg/mL respectively,with R reached 0.999 in both cases. The average recoveries were 111% and 99.7% respectively. The results of two methods determining 3 parallel samples were close. The error was allowed. By contrast,the UV spectrophotometry was much more simple and rapid,so it was more suitable for the determination of Vc in Maillard system.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第10期79-82,共4页
Science and Technology of Food Industry
基金
国家自然科学基金资助项目(31360408)
