摘要
使用Soxhlet提取装置,用氯仿、乙酸乙酯、丙酮、甲醇4种不同溶剂对沙棘籽分别进行8小时萃取。粗提物被用来进行抗氧化和抗菌活性筛选。在离体模型(清除DPPH自由基和脂质体模型系统)中的粉末减少和抗氧化活性评价显示在各种提取物中使用甲醇作为萃取剂的提取物具有最高的抗氧化活性。甲醇提取物也具有最高的抗菌活性。最低抑制浓度(MIC)评价显示,在加入甲醇提取物对蜡状芽孢杆菌、凝结芽孢杆菌、枯草芽孢杆菌、单核细胞增生利斯特菌、小肠结肠炎耶尔森氏菌进行培养,MIC浓度分别为200,300,300,300,和350ppm。研究结果表明,沙棘种子应用于医药和食品储藏。
Seabuckthorn （Hippophae rhamnoides L. form, ethyl acetate, acetone and methanol （MeOH） ） seeds were successively extracted with chlorousing a Soxhlet extractor for 8h each. The crude extracts were screened for antioxidant and antibacterialactivities. The reducing power and antioxidant activities evaluated in various in vitro models （1, 1-diphenyl-2-picrylhydrazine and liposome model system） showed the highest activity for MeOH extract. The MeOH extract was also found to posses maximum antibacterial activity. The MIC values, with respect to MeOH extract for Bacillus cereus, Bacillus coagulans , Bacillus subtilis , Listeria monocytogenes , Yersiniaenterocolitica , were found to be 200, 300, 300, 300, and 350 ppm, respectively. These results indicated the possibility of using seabuckthorn seeds for medicinal uses and food preservation.
关键词
沙棘
抗氧化活性
抗菌活性
种子提取物
甲醇提取
Hippophae rhamnoides
Antioxidant activity
Antibacterial activity
Seed extract
Methanolic extract
