摘要
目的探讨纳米银对人肺癌A549细胞毒性的影响及其作用机制。方法通过显微镜观察纳米银对A549细胞形态的变化。MTT法检测不同浓度纳米银对A549细胞增殖的影响。流式细胞仪检测纳米银对A549细胞周期阻滞的影响。Western blot和免疫组织化学法检测纳米银对A549细胞凋亡蛋白表达的影响。结果与对照组比较,随着纳米银药物浓度的增加,A549细胞抑制率显著增加,其半数抑制率为(74.23±4.17)mg/L。纳米银作用于A549细胞G2/M期,纳米银中、高浓度明显上调细胞周期调控p21、p53基因的表达;纳米银可抑制Bcl-2蛋白的表达,对caspase-3有活化作用。结论体外细胞实验证实,纳米银通过抑制A549细胞G2/M期,使p21、p53基因表达紊乱,诱导凋亡因子表达,通过多靶点作用抑制细胞生长。
Objective To investigate the effect of silver nanoparticles on the proliferation of human lung carcinoma A549 cells and its mechanism. Methods Cultured A549 ceils were treated with different concentrations of silver nanoparticles in vitro. The morphological change of A549 cells was observed by microscopy. Cell proliferation was determined by MTT assay. Cell cycle was examined by flow cytometry and the expression of p21, p53, Bcl-2 and caspase-3 proteins was detected by Western blot and immunohistochemistry. Results Compared with control group, the inhibition of A549 cells was significantly increased as the concentration of silver nanoparticles increased [ IC50 = （ 74.23 ± 4.17 ） mg/L ]. After silver nanoparticle treatment,cell cycle was arrested in Gz/M phase; the expression of p21 ,p53 and caspase-3 was increased and the expression of Bcl-2 was decreased. Conclusion Silver nanoparticles can inhibit the proliferation of A549 cells,which is associated with the blockage in G2/M phase, the up-regnlation of p21, p53 and caspase-3 expression, and the down-regulation of Bcl-2 expression.
出处
《实用肿瘤杂志》
CAS
2014年第2期148-152,共5页
Journal of Practical Oncology
关键词
细胞系
肿瘤
病理学
纳米结构
治疗应用
银
治疗应用
细胞增殖
药物作用
细胞凋亡
G2期
lung neoplasms/pathology
cell line, tumor/drug effects
cell line, tumor/pathology
nanostructures/ therapeutic use
silver/therapeutic use
cell proliferation/drug effects
apoptosis
G2 phase
