摘要
随机扩增多态性DNA(RAPD)是目前常用的DNA指纹图谱技术。采用改进的“ROSE法”提取DNA ,并建立了重复性较好的RAPD标准分析条件。在此标准条件下 ,检测了烟草 10个品种材料基因组的多态性。经 10个引物扩增 ,在得到的 53条扩增带中 ,有 10个片段为 10个材料所共有 ,多态性达81.1%。其中引物P7的扩增图谱 ,各品种间可以互相区别 ,差异明显 ,可用作烟草
Random amplified polymorphic DNA (RAPD) is a popular technique of fingerprinting. Good quality DNAs were extracted from 10 varieties accessions of tobacco by using improved “ROSE” method. Reproducible amplification products were obtained with rigorously optimized reaction conditions. Fifty\|three amplification fragments were generated by RAPD with 10 primers, among which 10 fragments were shared by all 10 accessions. The degree of polymorphism was 81.1%. RAPD patterns of 10 accessions generated by P 7 could be used as DNA fingerprint for identification of 10 tobacco varieties.
出处
《烟草科技》
EI
CAS
2001年第1期34-37,共4页
Tobacco Science & Technology
基金
四川省科委和四川省烟草公司科研基金资助课题
