摘要
本研究选取北京市 4个牛场共 731头健康荷斯坦奶牛 ,利用聚丙烯酰胺凝胶垂直板状电泳 (PAGE)对其乳蛋白进行分型 ,采用动物模型 (单基因位点模型和多基因位点模型 )来分析乳蛋白多态性与奶牛产奶性能间的关系 ,旨在证明乳蛋白多态性作为遗传标记进行辅助选择的可能性。动物模型遗传效应统计分析表明 :单基因位点模型中某些乳蛋白位点的效应不是乳蛋白本身的效应 ,而是连锁基因的效应。β- CN位点 B基因或连锁基因显著影响乳蛋白率、乳脂率 ;κ- CN位点和β- L g位点的 B基因或连锁基因分别对乳蛋白率。
A total of 731 Holstein cows from Beijing area was used to determine the genotypic and gene frequencies of milk protein types, and the relationships between milk protein loci and milk production traits. Milk samples were typed by polyacrylamide gel electrophoresis (PAGE). Effects of milk protein loci on milk performance were examined using animal model (singel gene analysis in which each milk protein locus was analysed separately and multigene analysis in which all milk protein loci were analysed simultaneously), aimed to demonstrate the possibility of milk protein polymorphism to conduct subsidiary selection as a genetic marker. The results of animal model indicated that some effects ascribed to certain milk protein genes in the single gene analysis were not effects of the milk protein gene itself but of linked genes. Variant B or a very closely linked gene of β casein, κ casein, β lactoglobulin affected percentages of milk protein and milk fat, respectively.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2000年第5期74-80,共7页
Journal of China Agricultural University
关键词
荷斯坦牛
乳蛋白
产奶性能
遗传标记
Holstein cows
milk protein
milk performance
animal model
genetic marker
