摘要
从山西某煤矿的酸性矿井水中分离得到菌株ATF-1,对其形态、生理生化特性、16SrDNA基因序列、生长特性及其对城市污泥的摇瓶沥滤效果进行了研究。结果表明:(1)该菌为革兰氏阴性菌,短杆状,经16SrDNA鉴定为嗜酸氧化亚铁硫杆菌(Acidthiobacillus ferrooxidans)。菌株ATF-1培养的最佳条件为初始pH 2.0、温度30℃、接种量10%(体积分数)、Fe2+投加量9.00g/L。(2)以菌株ATF-1作为沥滤菌处理城市污泥,经过15d的生物沥滤,污泥中Zn、Pb、Ni、Cu、Cd和Cr去除率的最大值分别为93.56%、46.54%、85.48%、97.68%、90.64%和45.15%,处理后污泥中的重金属含量符合《农用污泥中污染物控制标准》(GB4284—84)中规定的在酸性土壤上的最高容许含量。
A new strain of Acidithiobacillus ferroozidans ATF-1 is isolated from acid mine drainage of a coal mine in Shanxi Province. The morphology, biochemical and physiological characters, 16S rDNA sequence, growth char- acteristics of ATF-1 are then studied. And ATF-1 was inoculated into the sewage sludge to bioleach heavy metals. The result shows that the bacteria cell is rod-shaped, gram-negative and identified as Acidithiobacillus ferrooxidans. The optimum growth conditions for ATF-1 are initial pH of 2.0,culture temperature of 30 ℃,inoeulation ratio of 10%, and Fe2＋ dosage of 9. 00 g/L. The obtained strain ATF-1 was used as microbe of bioleaching to remove heavy metals from sewage sludge,after 15 d of bioleaching at 30℃ ,the maximum removal efficiency of Zn,Pb,Ni,Cu,Cd and Cr is 93.56% ,46.54% ,85.48%% ,97.68% ,90.64% and 45.15% ,respectively. The total contents of six heavy metals in the treated sludge could meet limited value of Chinese quality standards for sludge agricultural application （GB 4284-84）.
出处
《环境污染与防治》
CAS
CSCD
北大核心
2014年第3期1-7,13,共8页
Environmental Pollution & Control
基金
国家自然科学基金资助项目(No.41161075)
广西"八桂学者"建设工程专项经费资助项目
广西科学研究与技术开发计划项目(桂科攻11107021-6-1)
广西矿冶与环境科学实验中心资助项目(No.KH2012ZD004)
关键词
嗜酸氧化亚铁硫杆菌
生长特性
城市污泥
生物沥滤
Acidithiobacillus ferrooxidans
16S rDNA
growth characteristics
sewage sludge
bioleaching
