摘要
目的用寇氏法测定螺光黑壳菌酮A腹腔注射对昆明小鼠的半数致死量(median lethal dose,LD50)。方法实验动物为啮齿昆明小鼠,雌雄各半,体重(20±2)g。用序贯法摸索出受试物的全死剂量,而后设置5个剂量组,剂量比值r=1.25(或0.80)。一次性腹腔注射黑壳菌酮A后,连续观察10 d,统计各组死亡情况并计算半数致死剂量。结果各组小鼠均出现不同程度的腹腔刺激反应,雌性比雄性反应更强烈且呈剂量依赖性,30 min后刺激基本消失;受试物腹腔注射LD50为5.35 mg/kg,其95%可信区间为4.69～6.11 mg/kg。结论螺光黑壳菌酮A的LD50为5.35 mg/kg,死亡率呈剂量依赖性。
Objective Karber＇ s method was applied to test the median lethal dose （ LD50 ） of Spiropreussione A in KM mice by intraperitoneal injection. Methods KM mice which half is male and another half is female were used to measure 100% lethal dose by Sequential method first, the rest was divided into 5 groups randomly. The ratio of high dose group and low dose group is set up as 1.25 （ or 0. 8 ）. Then the KM mice were administered Spiropreussione A at different dosages through intraperitoneal injection. Toxicity symptoms and times of death of the mice were recorded, and LD50 of Spiropreussione A on mice was calculated. Results Different degrees of peritoneal irritation were observed in each group and it was more significant in female than male. The peritoneal irritation disappeared after 30 min. The LD50 of Spiropreussione A was 5.35mg/kg, 95% confidence interval was （4.69 -6. 11 ） mg/kg. Conclusion This experiment showed that the LD50 of Spiropreussione A is 5.35 mg/kg and the mortality is dose-dependent.
出处
《中国比较医学杂志》
CAS
2013年第7期25-28,共4页
Chinese Journal of Comparative Medicine
基金
"重大新药创制"科技重大专项
项目编号:2012ZX09301002-001-032
关键词
急性毒性
半数致死量
Spiropreussione A, Acute toxicity, Median lethal dose
