摘要
目的比较研究Up and Down法与寇氏法测定破伤风毒素LD50值。方法 NIH小鼠皮下注射破伤风毒素,分别使用寇氏法和Up and Down法计算LD50值。结果同一破伤风毒素经寇氏法计算得LD50值为4.97ng/Kg体重,Up and Down法计算LD50值为4.93 ng/Kg体重,95%置信区间为(4.71～5.13)ng/Kg体重。结论应用Up and Down法可测出与寇氏法相同结果的LD50值,且动物使用数量仅用8只。
Objective To compare the efficacy of Dixon and Mood up-and-down method and Karber method in determining tetanus toxin LD50 in mice.Methods Tetanus toxin in a dose of 0.5 mL at concentration of 0.329,0.311,0.295,0.280,0.267 or 0.256 ng/mL was subcutaneously injected into NIH mice.The LD50 of tetanus toxin was determined by Dixon and Mood up-and-down procedure and Karber test.Results The LD50 of tetanus toxin was 4.97 ng/kg body weight measured by Karber method,and 4.93 ng/Kg body weight by the up-and-down procedure.Conclusions Both Dixon and Mood up-and-down procedure and Karber test can achieve the same LD50 of tetanus toxin,but the up-and-down procedure is more simple and convenient,and 8 animals are enough for this test.
出处
《中国比较医学杂志》
CAS
2012年第12期28-30,共3页
Chinese Journal of Comparative Medicine
