摘要
为了探讨羊肚菌的液体发酵工艺,优化液体深层发酵培养基的配方。在单因素试验的基础,进行了多因素正交试验。结果表明,羊肚菌的液体深层发酵培养基最佳配方为:葡萄糖1.5%、玉米粉1.5%、蛋白胨0.3%、黄豆粉0.3%、KH2PO40.3%、MgSO40.2%、CaCO30.1%、VB10.001%,菌丝生物量达3.10 g·100mL-1;液体培养条件为pH值6.5左右、温度25℃。
In order to discuss technology of submerged fermentation of Morchella,the submerged culture medium formula was optimized.Based on single factor experiment,the orthogonal test was made.It is shown that the best formula were glucose 1 .5%,corn powder 1 .5%,peptone 0.3%,soybean powder 0.3%,KH2 PO4 0.3%,MgSO4 0.2%,CaCO3 0.1%,VB1 0.001%,the mycelium biomass could reach 3.1 0 g＆#183;1 00mL-1 .The submerged culture conditions were temperature 25℃ and pH 6.5 for Morehella esculenta.
出处
《包装与食品机械》
CAS
2014年第1期15-18,共4页
Packaging and Food Machinery
基金
安徽农业大学大学生科技创新基金项目(项目号:2012266)
关键词
羊肚菌
深层培养
工艺
菌丝生物量
Morehella esculenta
submerged culture
technology
mycelium biomass
