摘要
目的：建立补肾温肺合剂中黄芪甲苷的含量测定方法。方法：利用HPLC—ELSD法，采用AgilentZORBAXC18色谱柱（150mm×4．6mm，5μm）；流动相：甲醇-水（75：25）；漂移管温度：80℃；气体流速：2．5ml·min-1。结果：黄芪甲苷对照品在0．297—4．944μg范围内呈良好线性关系（r=0．9997），平均回收率为98．13％，RSD为1．27％（n=9）。结论：本方法结果准确，重复性好，可作为该制剂的含量测定方法。
Objective： To establish the content determination method for astragaloside in Bushen Wenfei mixtures by HPLC-ELSD. Methods： HPLC with an Agilent ZORBAX C18 （ 150 mm×4.6mm,5μm） column was used. Methanol-water（75： 25）was used as the mobile phase and the flow rate was 0.8 ml·min-1. The drift tube temperature was 80℃ and the gas flow rate was 2.5 ml ·min-1. Results： The linear range of astragaloside was 0.297-4. 944 I-Lg （ r = 0.999 7 ）. The average recovery was 98.13% with RSD of 1.27% （ n = 9）. Conclusion： The method is with sufficient accuracy, stability and repeatability, and can be used in determination of Bushen Wenfei mixtures.
出处
《中国药师》
CAS
2014年第2期322-324,共3页
China Pharmacist
