摘要
以河南南阳蜂胶为原料，采用反相高效液相色谱-二极管阵列检测器．电喷射串联质谱对蜂胶乙酸乙酯提取物进行分离鉴定。以10种黄酮对照品为分析对象建立分析方法，10种黄酮对照品的线性相关系数为0．9982～0．9999，加标回收率为93．40％103．69％，且精密度、稳定性和加标回收率的相对标准偏差均小于5％。采用该法对蜂胶乙酸乙酯提取物进行分析，河南蜂胶中可能含有槲皮素、芹菜素、山奈酚、异鼠李素、白杨素、松属素、高良姜素、短叶松素、短叶松素-3-乙酸酯、短叶松素-3-丙酸酯和短叶松素-3-丁酸酯11种黄酮类化合物以及对甲氧基肉桂酸肉桂酯和咖啡酸苯乙酯2种酚酸及其酯类化合物。
The purpose of this study is to separate and identify ethyl acetate extract of propoils from Nanyang, central China＇s Henan province by reverse phase-high performance liquid chromatography-diode array detector compled with electrospracy ionization-tandem mass spectrometry （RP-HPLC-DAD-ESI-MS-MS）. A method for detecting 10 flavonoid reference standards was established using RP-HPLC-DAD. The linear correlation coefficients for the ten flavonoids were 0.998 2--0.999 9 and the recoveries were 93.40%-103.69%. The RSDs of precision, stability, and spiked recovery were all less than 5%. The ethyl acetate extract of Henan pmpolis was analysed by RP-HPLC-DAD-ESI-MS-MS. Totally 11 flavonoids were identified including quercetin, apigenin, kaempferol, isorhamnetin, chrysin, pinocembrin, galangin, pinobanksin, pinobanksin- 3-O-acetate, pinobanksin-3-O-propionate, pinobanksin-3-O-butyrate and two phenolic acids and their esters.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2014年第2期186-190,共5页
Food Science
基金
中央高校基本科研业务费专项(2013PY100)
武汉市工程技术研究中心项目(200920137007)
关键词
蜂胶
反相高效液相色谱
质谱
黄酮
酚酸及其酯类
propolis
reverse phase-high performance liquid chromatography-mass spectrometry （RP-HPLC-MS）
flavonoids
phenolic acids and esters
