摘要
目的调查贵州地区静注海洛因成瘾者TT病毒感染状况。方法以公布的TTV第 1读码区序列设计两对寡核苷酸引物 ,用套式聚合酶链反应 (nPCR)检测 10 7例静注海洛因成瘾者血清中TTVDNA ,以 6 2例正常人为对照。同时用酶联免疫法则丙肝病毒抗体 (抗HCV) ,逆转录套式聚合酶链反应 (RT -nPCR)测庚肝病毒核糖核酸 (HGVRNA)。结果正常人TTVDNA阳性率 6 .45 %,海洛因成瘾者 2 5 .2 3 %。两组相比 ,差异有显著意义 (P <0 .0 0 5 )。成瘾者TTVDNA阳性率在男、女间无差异 ,与注毒时间长短无相关 (P >0 .5 )。 2 7例TTVDNA阳性成瘾者中 ,11例为单纯TTV感染 ,16例重叠HCV或HGV感染 ,其中TTV、HCV、HGV三重感染 8例 ,TTV重叠HCV5例 ,重叠HGV3例。结论贵州地区静注海洛因成瘾者中有TTV感染存在 ,感染率高低与性别、注毒时间长短无关。TTV可以单独感染 ,但与HCV、HGV的重叠感染率较高。
Objective To investigate TT virus infection in intravenous injection heroin drug users in Guizhou province. Methods A nested polymerase chain reaction (nPCR) assay with two-set of primers deduced from the first open read frame of TTV genome was established,the prevalence of TTV DNA was detected in 107 intravenous drug users (IVDUs) and 62 normal adults. The sera samples were also tested for antibody against hepatitis (anti-HCV antibody) and hepatitis G virus ribonucleotide acid (HGV RNA).Results The IVDUs of 25.23%(27/107) were TTV DNA positive,normal adults were 6.45% (4/62) postitve. 8 of 27 casess with TTV infection for both HGV RNA and anti-HCV positive,5 were positive for anti-HCV,3 were positive for HCV RNA. There was no relationship among TTV infection with sex,age,and persisted time of intravenous drug use in observed objects. Conclusion TTV infection should not be ignored in IVDUs,TTV super-infection with hepatitis C and hepatitis G appeared to be associated with a same transmitted-route among them.
出处
《贵州医药》
CAS
2001年第1期7-9,共3页
Guizhou Medical Journal
