摘要
目的 :研究苦参碱 (MT)和氧化苦参碱 (OMT)分别对二乙基亚硝胺 (DEN)诱发大鼠肝癌的预防阻断作用。方法 :采用 0 .0 1%的DEN诱发大鼠肝癌 90d ,同时分别腹腔注射MT和OMT注射液 15mg/kg ,停止诱癌及给药处理 30d后 ,处死大鼠 ,观察大鼠肝脏的病理改变、肝表面癌结节数、肝 /体重比和血清中丙氨酸氨基转移酶 (ALT)、γ 谷氨酰转肽酶 (γ GT)、碱性磷酸酶 (ALP)的变化。结果 :MT组和OMT组大鼠的体重明显高于模型组 ,肝表面癌结节数、肝 /体重比和血清ALT、γ GT明显低于模型组 (P<0 0 5) ,而ALP较模型组有所升高。另外 ,OMT组的肝重、肝 /体重比和血清ALP均明显低于MT组(P <0 0 5)。结论 :MT和OMT ,尤其是OMT ,尽管不能完全阻断DEN诱发大鼠肝癌的发生 ,但能保护肝细胞免受损伤 。
Objective:To study the preventive effect of Matrine(MT) and Oxymatrime(OMT)on rat hepatoma induced by diethylnitrosamine(DEN).Methds:Hepatoma rats induced by 0.01%DEN for 90 days,were administered with MT or OMT 15 mg/kg intraperitioneally at the same time.After 120 days,the rats were sacrificed and changes of hepatoma nodules,body weight,liver weight,the ratio of liver/body weight,were recorded and the serum alanine aminotransterase(ALT),γ glutamyl transferase(γ GT)and alkaline phosphatase(ALP) and the pathological changes of the livers were obseved.Results:In the MT group and OMT group,the body weight are larger,the hepatoma nodules,the ratio of liver/body weight and serum ALT,γ GT are lower,but serum ALP are higher than model group( P <0 05).Meanwhile,the liver weight,ratio of liver/body weight and ALP of OMT group are lower significantly than MT group( P <0.05).Conclusions:MT and OMT,especially OMT,can protect liver cells from damage induced by DEN and delay the forming of hepatoma,although MT and OMT can not inhibit the of hepatoma dcvelopirent completely.
出处
《肿瘤防治杂志》
2000年第6期561-563,共3页
China Journal of Cancer Prevention and Treatment
基金
"95"军队医药卫生科研基金! ( 98D0 42 )
