摘要
目的 :研究珍珠梅提取物对二乙基亚硝胺 (diethylnitrosamine,DEN)致大鼠肝脏癌前病变的抑制作用。方法 :给动物ig给药 ,用DEN制备大鼠肝脏癌前病变模型 ;用免疫组织化学S P法检测大鼠肝脏谷胱甘肽S 转移酶 (GST P)的表达水平 ,用比色分析法检测血清、肝匀浆、线粒体SOD、GSH Px的活性及MDA含量的变化。结果 :珍珠梅提取物能显著降低DEN致癌前病变大鼠肝脏GST P的表达 ,升高血清、肝匀浆、肝线粒体SOD、GSH Px的活性 ,降低MDA的含量。结论
Objective:To investigate the inhibitory effect of Sorbaria Sorbifolia extract on diethylnitrosamine (DEN) induced precancerous hepatic in rats.Methods: Sorbaria Sorbifolia was administrated ig. to rats with DEN\|induced precarcinogenesis,Then using the immunohistochemical S\|P method,the p53 and bcl\|2 proteins were detected.The activities of superoxide dismutase(SOD),glutathione peroxidase (GSH\|Px),and the concentrations of malondialdehyde(MDA) were investigated by colorimetric method.Results: Sorbaria Sorbifolia significantly decreased the expression of GST\|P,and increased the SOD,GSH\|Px and decreased MDA level in serum,liver cell homogenate and liver cell mitochondria.Conclusion: Sorbaria Sorbifolia extract has inhibitory effect on DEN\|induced precancerous hepatic in rats.
出处
《中国实验方剂学杂志》
CAS
2004年第4期33-35,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
吉林省延边朝鲜族科技发展计划项目 (YBKJ0 2 7)
关键词
珍珠梅
肝肿瘤
化学诱导
二乙基亚硝胺
毒性
Sorbaria Sorbifolia
liver neoplasm/chemically induced
diethylnitrosamine/toxicity
