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8起副溶血性弧菌食物中毒分子流行病学特征分析 被引量：7

Molecular epidemiology traits of 8 Vibrio parahaemolyticus food poisoning incidents
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摘要 目的调查并分析镇江及周边部分地区8起副溶血性弧菌食物中毒的分子流行病学特征。方法对经API生化鉴定的40株副溶血性弧菌进行毒力基因(tdh和trh)及功能基因(toxRS/new和orf8)测定、血清学分型和脉冲场凝胶电泳(PFGE)分型。结果 tdh阳性率为85.0%(34/40),trh阳性率为5.0%(2/40),toxRS/new阳性率为50.0%(20/40),orf8阳性率为42.5%(17/40);血清型以O3∶K6为主;PFGE分型显示A^B群间相似度>80%。结论镇江及周边部分地区8起食物中毒事件中的副溶血性弧菌主要为O3∶K6大流行克隆,且具有高度同源性。 Objective To analyze the molecular epidemiology traits of Vibrio parahaemolyticu. in Zhenjiang and surrounding areas. Methods 40 Vibriu parahaemolyticus strains were collected from the samples of food poison incidents occurred in Zhenjiang and surrounding areas in 2011. Biochemical identification, tdh, trh and toxRS/new gene detection, serotyping and pulse-field gel electrophoresis （PFGE） were applied to these strains. Results The positive rate of tclh, trh, toxRS/new and orJ8 were 85.0% , 5.0% , 50. 0% and 42. 5% , respectively. The prevalent serotype was O3：K6 （32/40）. The similarity of PFGE type between group A and B was more than 80%. Conclusion Strains from different regions in Zhenjiang and surrounding areas were mainly O3：K6 pandemic clone and highly homologous.
作者 刘丽萍 徐岚 李薇薇 裴晓燕 郭云昌
机构地区 镇江市疾病预防控制中心 国家食品安全风险评估中心
出处 《中国食品卫生杂志》 北大核心 2014年第1期9-13,共5页 Chinese Journal of Food Hygiene
基金 江苏省自然科学基金项目(BK2010350) 镇江市卫生科技重点专项项目(SH2011068)
关键词 副溶血性弧菌 血清型 O3:K6大流行克隆 脉冲场凝胶电泳 食源性致病菌 食物中毒 Vibrio parahaemolyticus serotype O3 ： K6 pandemic clone pulse-field gel electrophoresis food-borne pathogens food poisoning
分类号 R155.3 [医药卫生—营养与食品卫生学] R181.2 [医药卫生—流行病学]
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参考文献14

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二级参考文献16

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中国食品卫生杂志
2014年 第1期

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