摘要
目的 探讨硒对淋巴细胞抗大肠癌过程中凋亡相关基因Fas/FasL表达的影响。方法 以半胱氨酸硒作为影响因素 ,人T淋巴细胞为效应细胞及人结肠癌细胞 (LoVo细胞株 )为靶细胞 ,采用四甲基偶氮唑蓝 (MTT)比色法、凋亡细胞荧光计数检测凋亡细胞的数量 ,逆转录 聚合酶链 (RT PCR)反应、原位杂交技术检测 ,硒对效应细胞杀伤肿瘤细胞过程中Fas/FasL表达的影响。结果 不同浓度的半胱氨酸硒 (0 .5、1.0mg/L)作用 48h后 ,可增强人T淋巴细胞抗肿瘤活性 [分别为 (2 5 .12± 3 .91) % ,(46 .17± 3 .6 8) % ,P <0 .0 5 ] ,且肿瘤细胞的凋亡比例上升 [分别为(18.6± 4.1) % ,(32 .7± 2 .1) % ,P <0 .0 5 ] ;能使免疫效应细胞和肿瘤细胞表达FasL和Fas水平增高。结论 硒可提高淋巴细胞的抗肿瘤作用 。
Objective To observe the effects of selenium on the expression of Fas/FasL in T lymphocyte anti colonic cancer cells. Methods Selenocysteine served as an influencing factor, T lymphocyte as an effector and human colonic tumor cell (LoVo cell: human colonic adenocarcinoma) as a target. MTT and acridine orange dying were used to measure LoVo cells apoptosis, and RT PCR and hybridization techniques used to identify the levels of Fas/FasL and TNF expressed by T cells or target cells after use of selenium or not. Results MTT and acridine orange dying showed that selenium enhanced T lymphocyte anti tumor function [(25.12±3.91)%,(46.17±3.68)% respectively,( P <0.05)],and induced LoVo cells apoptosis and this effect was [ (18.6±4.1)%, (32.7±2.1)% respectively,( P <0.05)] at the proper concentration (0.5～1.0 mg/L) in time and dose dependent manners. And the levels of FasL and Fas mRNA expressed by target cells (LoVo cell) and effectors were increased. Conclusion Selenium enhanced T lymphocyte anti tumor function, which was related to high levels of Fas/FasL expression. [
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2001年第1期21-22,共2页
Chinese Journal of Experimental Surgery
