摘要
目的探讨硒对淋巴细胞抗结肠癌细胞作用的影响。方法我们以半胱氨酸硒作为影响因素,人T淋巴细胞为效应细胞及人结肠癌细胞(Lovo细胞株)为靶细胞,采用MTT比色法、凋亡细胞荧光计数检测凋亡细胞的数量,并检测上清液中TNFα,SOD和MDA的变化。结果半胱氨酸硒在一定的浓度范围内(0.5mg/L～1.0mg/L),可增强人T淋巴细胞抗肿瘤活性(P=0.042),促进人结肠癌细胞的凋亡,且肿瘤细胞的凋亡比例上升(P=0.006);T淋巴细胞抗肿瘤作用的增强与上清液种中的TNF-α的增高有关(P=0.01),但与SOD和MDA无关(P>0.05)。结论硒可提高淋巴细胞的抗肿瘤作用,且与TNF-α有关。
AIM To study whether selenocysteine could enhance T lymphocyte anti-colonic cancer cell function. METHODS Selenocysteine was tested as an influencing factor to human T lymphocyte in human colonic tumor cells (Lovo cell: human colonic adenocarcinoma) as a target cell. There were four groups: control, Se0.5, Sel.O,Se2.0(mg/L). The ratio of effector cell target cell was 20 : 1. MTT and acridine orange for quantitative measurement were used. TNFα, SOD and MDA were also measured.RESULTS MTT and acridine orange dying showed that selenium enhanced T lymphocyte anti-tumor function and induced Lovo cells to apoptosis within the proper levels (0.5 g/ L-1.0 g/ L), and this effect was time and dose-dependent (P =0.0006). Enhancement of T lymphocyte anti-tumor function is related to TNFα (P = 0.01), and not to SOD and MDA (P>0.05).CONCLUSION Selenocysteine can enhance T lymphocyte anti-colonic cancer function, which is also related to TNFα, and not to SOD and MDA.
出处
《世界华人消化杂志》
CAS
2000年第1期80-83,共4页
World Chinese Journal of Digestology
关键词
硒
淋巴细胞
细胞凋亡
大肠癌
selenium
lymphocyte
colorectal neoplasms
apoptosis
