摘要
目的 观察谷氨酰胺对内毒素血症大鼠小肠粘膜抗氧化损伤的保护作用。方法 2 8只大鼠随机分为 3组 ,A组 :谷氨酰胺 (Gln)肠外营养 (PN)组 (n =10 ) ;B组 :不含Gln的常规TPN组 (n =10 ) ;C组为正常对照组 (n =8)。内毒素以 2mg/ (kg·d)的剂量混入营养液或等渗盐水中 ,持续滴注 5天。实验结束处死动物 ,取小肠粘膜测定蛋白质、DNA、ATP、超氧化物歧化酶 (SOD)、丙二醛 (MDA)、谷胱甘肽 (GSH)和分泌型免疫球蛋白A(sIgA)的含量。结果 内毒素血症大鼠上述指标除MDA外 ,均明显低于正常对照组 ,P <0 0 5 ;A组空回肠壁蛋白质、DNA、ATP、SOD、GSH及sIgA含量均高于B组 ,P <0 0 5 ;MDA含量明显低于B组 ,P <0 0 5。结论 加入Gln的TPN有助于防止内毒素血症大鼠肠粘膜的氧自由基损伤 ,其机理可能是通过增加GSH的途径而实现的。
Objective\ To study the protective effect of glutamine on the intestinal mucosal antioxidation in endotoxemic rats. Methods Twenty eight male Wistar rats were randomly divided into three groups, group A:parenteral nutrition supplemented with glutamine, group B:TPN without glutamine,and group C:normal control. Endotoxemia was induced by continous intravenous infusion of lipopolysaccharide(LPS) at a dose of 2 mg/kg per day throughout the 5 day study period. The mucosal protein、DNA、ATP、SOD、MDA、GSH、sIgA were determined. Results\ The mucosal protein、DNA、ATP、SOD、GSH and sIgA content in endotoxic rats were markedly decreased, MDA was increased as compared with normal control( P<0.05) . The former indices in group A were improved and MDA content was decreased as compared with group B( P<0 05) . Conclusion Glutamine can improve gut energy metabolism, decrease the extent of mucosal injury of free radicals, and give an protective effect on the mucosal probably by increasing GSH.
出处
《中国普外基础与临床杂志》
CAS
2001年第1期5-7,共3页
Chinese Journal of Bases and Clinics In General Surgery
基金
上海市青年科技启明星计划资助!课题 (99QB1 4 0 1 0 )
