摘要
目的 探讨丙氨酰 谷氨酰胺二肽 (Ala Gln)对肝脏缺血再灌注损伤 (HIRI)的保护作用。方法 采用大鼠HIRI模型 (Pringle’s法阻断入肝血流 30min) ,分谷氨酰胺组 (G组 )及对照组(C组 ) ,检测再灌注后血清肝生化酶、肝组织还原型谷胱甘肽 (GSH )及超氧化物歧化酶 (SOD)水平 ,对肝组织进行光镜与电镜检查 ,并计算术后 2 4h生存率。结果 再灌注 1h ,G组血清ALT(4 99 2 5±12 0 84 )U /L、LDH(6 95 6 0 0± 2 4 4 3 93)U/L的水平均显著低于C组 (ALT :82 3 5 6± 32 8 71,P <0 0 5 ;LDH :11715 31± 2 993 5 0 ,P <0 0 1) ;再灌注 2 4h ,两组血清ALT、LDH的水平均有显著恢复 ,但G组 (ALT :176 6 9± 15 1 84 ;LDH :4 15 38± 2 13 6 8)水平仍显著低于C组 (ALT :5 4 8 2 5±2 5 7 2 5 ;LDH :195 8 5 0± 6 87 32 ;P <0 0 1)。再灌注 1h及 2 4h ,G组GSH的水平分别为 (12 16 0 9± 15 2 78) μg·g-1·p、(899 73± 5 7 75 ) μg·g-1·p ,均明显高于C组 (分别为 85 6 6 8± 117 6 4 ,P <0 0 1;80 0 5 0± 94 79,P <0 0 5 ) ;两组SOD的活性无统计学差异。G组肝脏组织学与细胞学损害均明显轻于C组。G组术后 2 4h生存率为 78 5 7% (11/ 14 ) ,明显高于C组的 4 5 4 5 % (10 / 2 2 ) (P <0 0 5 )?
Objective To investigate the protective effect of alanyl glutamine dipeptide (Ala Gln) against hepatic ischemia reperfusion injury (HIRI) in the rats. Methods Hepatic inflow was occluded for 30 minutes by Pringle's method to estabolish HIRI model. The rats were devided into glutamine (G) and control (C) groups. After reperfusion, liver enzymes, glutathione of reduced type (GSH), superoxide dismutase (SOD) of liver tissue and pathological changes of liver were examined. Twenty four hour survival rate after operation was calculated. Results At 1 h post reperfusion, liver enzymes of G group were significantly lower than those of C group, respectively. [ALT ( 499 25±120 84)U/L vs (823 56±328 71)U/L, P<0 05; LDH ( 6 956 00±2 443 93)U/L vs (11 715 31±2 993 50)U/L,P<0 01]. At 24 h post reperfusion, liver enzyme levels of both groups markedly recovered and the levels of G group were also significantly lower than those of C group, respectively. [ALT (176 69±151 84)U/L vs (548 25±257 25)U/L, P<0 01; LDH (415 38±213 68)U/L vs (1 958 50±687 32)U/L, P<0 01]. At 1 and 24 hours after reperfusion, the levels of GSH (μg·g -1 ,prot)of G group was obviously higher than that of C group, respectively (1 216 09±152 78 vs 856 68±117 64, P<0 01; 899 73±57 75 vs 800 50±94 79, P<0 05). There was no statistical difference in the activity of SOD between two groups. Hepatic pathological changes of G group were more serve than those of C group. The 24 hour survival after operation of G group was 78 57%(11/14), higher than that of C group 45 45%(10/22) (P<0 05). Conclusions Ala-Gln (Gln) has protective effects against HIRI and this effect was mediated of least in part by maintaining the stablity GSH in liver tissue
出处
《消化外科》
CSCD
2004年第4期261-266,共6页
Journal of Digestive Surgery
基金
辽宁省自然科学基金资助课题
