摘要
目的探讨阿魏酸钠在糖尿病大鼠阴茎勃起功能障碍中的干预作用及其机制。方法选择雄性SD大鼠通过腹腔内注射链脲佐菌素方法建立糖尿病大鼠模型44只，然后将成模大鼠分成糖尿病干预组22只和糖尿病对照组22只，并设正常对照组。其中糖尿病干预组每日灌胃法给予阿魏酸钠100mg·kg^-1·d^-1干预。12周后观察大鼠阴茎勃起功能，并获取阴茎海绵体组织和血清中的超氧化物歧化酶（SOD），过氧化氢酶（CAT），一氧化氮合酶（NOS）与丙二醛（MDA）的水平变化和阴茎海绵体组织的病理学改变。结果成功建立糖尿病大鼠模型。与糖尿病对照组相比，糖尿病干预组和正常对照组大鼠阴茎勃起功能更好，3组的阴茎勃起率分别为22％、66％和100％。SOD、CAT和NOS在糖尿病射照组中的活性均低于正常对照组和糖尿病下预组；NO在正常对照组和糖尿病干预组的含量[（112±28）nmol／ml、（137±25）nmol／m1]高于糖尿病对照组[（56±10）nmol／ml，均P〈0．01]；MDA在正常对照组和糖尿病干预组的含量低于糖尿病对照组（均P〈0．01）。光镜下，糖尿病对照组阴茎海绵体平滑肌数量减少，间质血管管壁增厚，管腔变窄。结论阿魏酸钠可能通过调节自由基代谢、抗氧化损伤和促进NO生成增加的途径，对糖尿病性勃起功能障碍起到下预作用。
Objective To investigate the effect and mechanism of sodium fern!ate （SF） on reversing erectile dysfunction in diabetes mellitus （DM） rats, Methods Forty-four male adult Sprague- Dawley rats were induced for diabetes by an intraperitoneal injection of streptozotocin. Then the successful models were randomly divided into DM ＋ SF group and DM group（22 rats each respectively）. The rats in DM ＋ SF group were treated with sodium ferulate （ 100 mg·kg^-1· d^-1 ） through a daily gastric lavage. At Week 12, the erectile function of all rats was evaluated and serum samples were harvested. The SOD, CAT, NOS, MDA and NO levels in corpus cavernosum and serum were all measured. The pathologic change in penile cavernous body was observed microscopically. Results. The diabetic rat models were successfully established. The erectile function was much better in normal control group and DM ＋ SF group than that in DM group. And the penile erection rates in three groups were 100% , 66% and 22% respectively. The activity levels of SOD, CAT and NOS were markedly decreased in DM group as compared to those in normal control group and DM ＋ SF group （P 〈 0. 01 ）. The NO content was approximately equal in normal control group and DM ＋ SF group（ 112 ± 28）nmol/ml vs （137 ± 25 ）nmol/mL But both were much higher than that in DM group（56 ± 10）nmol/ml,both P 〈 0. 01. The MDA content was markedly increased in DM group as compared to those in normal control group and DM ＋ SF group （both P 〈 0. 01 ）. Microscopically, muscle fibers in penile cavernous body arranged disorderly, muscular mantle damaged and desmoplasia scattered among muscle fibers in DM group. Conclusion Sodium ferulate may play interventional roles in reversing diabetic erectile dysfunction through metabolic regulation of free radicals, antagonism of oxidative insults and enhancement of NO production.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2009年第24期1711-1713,共3页
National Medical Journal of China
基金
浙江省科技厅基金（2005C30063）
浙江省中医约管理局青年科研基金（2006Y007）
关键词
阳萎
糖尿病
一氧化氮合酶
阿魏酸钠
Impotence
Diabetes mellitus
Nitric oxide synthase
Sodium ferulate
