摘要
目的通过对巨结肠小肠结肠炎、无肠炎患儿及正常儿童大便中双歧杆菌和乳酸杆菌进行检测,初步探讨乳酸菌群和先天性巨结肠小肠结肠炎的关系。方法选取2006年1月至2006年12月在上海交通大学医学院附属新华医院行先天性巨结肠根治术的患儿共30例,1个月～7岁,平均年龄1.2岁。根据术前术后有无小肠结肠炎临床症状分为HD小肠结肠炎组和HD非肠炎组,其中肠炎10例。并选同龄儿10例为正常对照组。分别采集各组的新鲜大便,立即置入无菌离心管,-20℃冻存,24 h内抽提粪便内细菌基因组DNA。结果由于粪便内双歧杆菌和乳酸杆菌绝对值不呈正态分布,故取其对数值(log_(10))进行方差分析。各组双歧杆菌对数含量:巨结肠肠炎组7.07±0.85和巨结肠非肠炎组8.36±0.80、正常对照组8.46±0.92比较,差异均有统计学意义(P<0.05)。巨结肠非肠炎组和正常对照组相似,差异无统计学意义(P>0.05)。各组乳酸杆菌对数含量:巨结肠肠炎组5.51±0.65、巨结肠非肠炎组5.79±0.82,分别和正常对照组6.47±0.71比较,差异有统计学意义(P<0.05)。结论结合本实验结果提示在巨结肠患儿中双歧杆菌和乳酸杆菌的含量明显低于正常组,这可能是肠炎发生的原因之一。
Objective To detect the bifidobacterium and lactobacillus in children with Hirschsprungs disease （HD） associated with enterocolitis. Methods Between January 2006 and December 2006, thirty cases of HD with age ranging from 1 month to 7 years（mean= 1. 2 year） were included in this study. According the clinic patterns of enterocolitis, the patients were divided into enterocolitis group（n = 10） and non-enterocolitis group（n = 20）. Real-time quantitative PCR were used for detection of bifidobacterium and lactobacillus in the stool. Results The bifidobacterium in HD associated enterocolitis was 7. 07 ±0. 85, which was significant lower than that in HD（8. 46 ± 0. 92） or normal controls（8. 36 ± 0. 80）. Similarly, the lactobacillus in HD associated enterocolitis was 5.51 ± 0. 65. which was lower than that in HD group （ 5.79 ± 0. 82） or controls （6. 47 ± 0.71 ）. Conclusions These results suggest that decreased bifidohacterium and lactobacillus in the intestine may play an important role in the cause of HD associated enterocolitis.
出处
《中华小儿外科杂志》
CSCD
北大核心
2007年第7期351-354,共4页
Chinese Journal of Pediatric Surgery
关键词
巨结肠
先天性
小肠结肠炎
双歧杆菌
Hirschsprung＇s diseases Enterocolitis, Bifidobacterium
