摘要
目的 :从接种新城疫F48E9病毒的LaSota疫苗免疫鸡体外有丝分裂原刺激的脾淋巴细胞中克隆鸡干扰素基因。方法 :应用逆转录多聚酶链式反应技术扩增 ,常规平端连接、转化及序列测定技术进行验证。结果 :研究表明克隆得到的基因与国外报道的鸡胚成纤维细胞干扰素基因。结论
Objective:To clone the gene of chicken interferon(IFN) from splenocytes of Newcastle disease virus(NDV) La Sota vaccinated chickens which then injected with NDV F48E9 strain.Methods:The techniques of RT PCR,convenient ligation,transformation and sequencing were used.Results:The sequence of cloned gene was just the same as the published chicken embry cells IFN.Conclusion:The chicken embry cells IFN mRNA was expressed in splenocytes of Newcastle disease virus(NDV) La Sota vaccinated chickens which then injected with NDV F48E9 strain.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2001年第1期45-47,共3页
Chinese Journal of Immunology
基金
农业部"九五"生物技术攻关项目资助
