摘要
将50、100和200μgpcDNA—SDIFN—α分别肌肉注射28日龄樱桃谷肉鸭，设PBS和空载体质粒pcDNA3．1（＋）对照，建立并应用TaqMan探针实时荧光定量PCR检测了不同时间pcDNA—SDIFN—α在血液以及各组织器官中的含量。结果，建立的PCR具有特异性强、灵敏度高、快速等特点，各剂量pcDNA—SDIFN—α给鸭免疫后的第1h即可在血液及各组织中检测到，注射部位显著高于除心脏外的其他组织；除血液外其他器官中pcDNA—SDIFN—α的含量在1～24h达到最高，随后逐渐减少；105d时各组织仍能检测到（约1×10^2拷贝／g）。不同剂量pcDNA—SDIFN-α在免疫鸭各组织中的含量的总体规律为200μg〉100μg〉50μg。表明，pcDNA—SDIFN—α肌肉注射能迅速分布到鸭体各组织器官中并持续存在105d以上。
Five groups of 28-day-old ducks were injected intramuscularly with 50,100 and 200 μg of pcDNA-SDIFN-α, respectively, with PBS and pcDNA3.1 （＋） as controls. A real-time fluorescent quantitative PCR was established and applied to detect the copy numbers of pcDNA-SDIFN-α in peripheral blood and organs at different time points post-injection（PI）. The established PCR was specific, highly sensitive, and rapid for detecting pcDNA-SDIFN-α. The DNA vaccine pcDNA-SDIFN-α could be detected in all tissues and peripheral blood at hour 1 PI. The copy numbers of pcDNA-SDIFN-α at the injection site was significantly more than those at other samples except the heart. The copy numbers of pcDNA-SDIFN-α at each tissue reached peak at hour 1 to 24 PI, then it began to decrease. However,on day 105 PI,it could still be detected in all the tissues, but the copy numbers were only 1 ×10^2 copies per gram. It was concluded that the DNA vaccine pcDNA-SDIFN-α could be quickly distributed in every tissue after being injected intramuscularly,and it could be in existence for more than 105 d.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2007年第5期390-395,共6页
Chinese Veterinary Science
基金
国家科技攻关重大项目(2004BA901A03)
教育部"新世纪优秀人才支持计划"项目(NCET-04-0906)
四川省重大基础研究项目(05JY029-109)
四川省重点项目(2003A024)
四川省重点建设学科项目(SZD0418)
