摘要
对ＬａＳｏｔａ免疫雏鸡人工感染新城疫强毒前后Ｔ淋巴细胞免疫活性和血清ＩｇＧ抗体含量的动态变化进行了检测。实验表明，ＬａＳｏｔａ免疫鸡在强毒攻击前脾淋巴细胞对ＣｏｎＡ和ＰＨＡ均无反应性，但血清ＩｇＧ抗体的含量则逐渐升高。在受到强毒攻击后，脾淋巴细胞对ＣｏｎＡ和ＰＨＡ具有非常明显的反应性，而血清ＩｇＧ抗体起初下降，随后逐渐升高，这些结果揭示在新城疫的免疫和感染中，细胞免疫和体液免疫具有协同作用。
Blastogenesis microassay employing 3(4,5dimethylthiazol2yl)2,5diphenyl tetrazolium bromide (MTT)and ELISA were adapted to measure blastogenic respones of lymphocytes and serum IgG antibody respectively from La Sotaimmuned and F48E9 incubated La Sotaimmuned chickens,It is demonstrated that the lymphocytes of La Sotaimmuned chickens have low respone to ConA and PHA,but have high respone when they incudated with F48E9 strain.The serum IgG antibody increased gradually when the chickens immunized with La Sota vaccine,but decreased significantly when they incubated with F48E9 strain. These results indicate that the cellular immune response,in synergy with humoral immune respone,plays a critical role in resistence to Newcastle disease virs(NDV)infection.
关键词
新城疫
细胞免疫
体液免疫
鸡病
Newcastle disease Cellular immune Humoral immune
