摘要
目的 :探讨内皮源性白细胞介素 8(IL 8)对人树突状细胞 (DC)的作用。方法 :用TNF α分别诱导内皮细胞和单核细胞产生IL 8,并将这 2种不同来源的IL 8分别加入DC常规诱导培养的早期 (第 3天 )及后期 (第 7天 ) ,培养至第 9天收获细胞。采用流式细胞术 (FCM)分析DC的表型 ;体外混合淋巴细胞反应 (MLR)测定DC刺激T细胞增殖能力 ;ELISA检测培养上清中IL 12的含量 ;PI染色观察DC凋亡。结果 :在DC培养的早期 (第 3天 )加入内皮源性IL 8可以抑制DC的成熟 ,FCM分析表明 :CD1a ,CD40 ,CD80 ,CD83 ,HLA DR等DC表面标志显著下降 ,而CD14表达率明显高于常规培养组。同时 ,激发同种T细胞增殖的能力及分泌的IL 12量均显著低于常规组 (P <0 0 1) ,而加入单核细胞来源的IL 8则无此效应。两种细胞来源的IL 8均不会引起DC凋亡。结论 :内皮细胞源性IL 8可以抑制体外培养早期DC的成熟 ,促使其向单核
Objective:To explore the impact of endothelial interleukin 8(IL 8) on human dendritic cells Methods: Human endothelial cells and monocytes can produce and secrete IL 8 respectively by stimulation of TNF α These two types of IL 8 of different origins were added to the routine DC culture medium at an early stage (3rd day) or at a late stage (7th day) mataining till cells were collected at 9th day Cell differentiation and function were evaluated by flow cytometry(FCM) , mixed lymphocyte reaction(MLR), detection of IL 12 of supernernt by using ELISA Apoptosis was observed by PI staining Results: Endothelial IL 8 which if added at an early stage (3rd day) to DC culture could inhibit DC maturation and there showed greatly decreased levels DC markers:CD1a,CD40,CD80,CD83 and HLA DR, while with CD14 expression obviously up regulated This inhibitory effects were also demonstrated in a reduced allogenenic MLR and IL 12 secretion Monocytic IL 8 does not have such negative impact on DCs Neither endothelial IL 8 nor monocytic IL 8 would induce DC apoptosis Conclusion:Endothelial IL 8 added to DC culture at an early stage could inhibit DC maturation and may conduct the macrophage lineage differentiation
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2001年第1期3-6,共4页
Chinese Journal of Immunology
基金
国家自然科学基金资助!(396 2 5 0 2 5 4)
杰出青年基金资助!(2 96 2 5 0 2 4)
