摘要
目的 探讨白细胞介素（IL）-8基因多态性与矽肺易感性的关系.方法 选择确诊的101例矽肺患者为病例组,以接触同性质粉尘、首次诊断年龄相近的非矽肺的接尘工人为对照（121例）.采集外周静脉血,盐析法提取DNA,应用聚合酶链-限制性片段长度多态性（PCR-RFLP）技术检测IL-8（Met31Arg、781C/T、-251A/T、RA＋860）4个位点的基因型和等位基因频率并进行分析.结果 两组对象的首次诊断年龄、累积接尘工龄、吸烟率的差异均无统计学意义（P＞0.10）.病例组IL-8（Met31Arg）GT基因型的分布频率为12.87%,对照组为2.48%,差异有统计学意义（P＜0.05）,病例组和对照组携带等位基因G的频率分别为6.44%和2.07%,差异有统计学意义（P＜0.05）.病例组IL-8（-251A/T）从基因型的分布频率为9.90%,对照组为25.62%,差异有统计学意义（P＜0.05）.病例组IL-8（781C/T）基因型CC、CT、TT的分布频率分别为38.61%、40.59%、20.79%,与对照组（46.28%、40.50%、13.22%）相比,差异无统计学意义（P＞0.05）.病例组IL-8（RA＋860）基因型GG、GC、CC的分布频率分别为75.25%、21.78%、2.97%,与对照组（80.17%、14.88%、4.96%）相比,差异无统计学意义（P＞0.05）.结论 IL-8的Met31Arg位点和一251MT位点的基因多态性与矽肺易感性有关,携带IL-8（Met31Arg）GT基因型的接尘工人患矽肺的危险性增加;携带IL-8（-251）从基因型的接尘工人患矽肺的危险性降低.未发现IL-8（781C/T和RA＋860）位点的基因多态性与矽肺易感性有关.
Objective To explore the relationship between the polymorphisms of interleukin-8（IL-8）and the silicosis susceptibility. Methods The case group consisted of 101 male patients with stage I silicosis diagnosed by the Pneumoconiosis Diagnosis Expert Panel according to the Chinese National Diagnosis Criteria of Pneumoconiosis （GBZ 70-2009）. The control group consisted of 121 workers without silicosis exposed to same dusts. The cases and the controls had the same dust exposure history. The peripheral venous blood was drawn from each subject. DNA was extracted from leucocytes by the salting method. The polymerase chain reaction restriction fragment length polymorphism （PCR-RFLP） techniques and PCR were used to examine polymorphism of IL-8（Met31Arg, 781C/T,-251A/T and RA＋860）. Results There were no the differences of age, cumulative exposure time and smoking between the cases and the controls （P〉0.05）. The frequencies of IL-8 （Met31Arg） GT genotypes in cases and controls were 12.87% and 2.48%, respectively, there was significant difference （P〈0.05）.The frequencies of allele G in cases and controls were 6.44% and 2.07%, respectively, there was significant difference （P〈0.05）. The frequencies of IL-8 （-251A/T） AA genotypes in cases and controls were 9.90% and 25.64%, respectively, there was significant difference （P〈0.05）. The frequencies of IL-8 （781C/T） CC, CT, TT genotypes in cases and controls were 38.61%,40.59%,20.79% and 46.28%,40.50%, 13.22%, respectively, there zwas no significant difference （P〉0.05）. The frequencies of IL-8 （RA＋860） GG, GC and CC genotypes in cases and controls were 75.25%, 21.78%, 2.97%, 80.17%, 14.88%, 4.96%, respectively, there was no significant difference （P〉0.05）. Conclusions IL-8 （Met31 Arg and -251A/T） genetic polymorphisms might play a role in the development of silicosis. The risk of pneumoconiosis in workers carrying （Met31 Arg） genotype GT is likely to increase.The risk of pneumoconiosis in workers carrying IL-8 （-251A/T） AA genotype is likely to decrease. The relationship between IL-8 781C/T and RA＋860 genes polymorphisms and silicosis is not found.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2011年第7期522-524,共3页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
河北省科技厅支撑项目（09276195D）
关键词
矽肺
白细胞介素-8
多态性
单核苷酸
易感性
Silicosis
Interleukin-8
Polymorphism, single nucleotide
Disease susceptibility
