摘要
目的：以测定血清中ＣＡ１９－９含量为例，介绍化学发光分析方法在临床检测中的应用并与放射免疫测定进行比较。方法：用化学发光仪对浓度为４１１．９ｎｇ／ｍｌ及６６４．５ｎｇ／ｍｌ的ＣＡ１９－９样品做１：２，１：４，１：８，１：１６稀释回收试验，计算百分回收率；测定批内变异、批间变异（％）；用化学发光仪及ＣＩＳ公司的放免药盒测定８１份样品中的ＣＡ１９－９含量，其中５２份样品用ＤＰＣ公司的放免药盒测定，计算化学发光分析与放射免疫分析所得结果的相关性，并求出回归方程。结果：稀释试验回收百分率为１０１．７％～１１５．４％。化学发光分析测定ＣＡ１９－９批内变异及批间变异均小于５％。化学发光法与用ＣＩＳ放免药盒测定的ＣＡ１９－９结果进行相关分析，相关系数为０．８９，回归方程为Ｙ＝１．８３Ｘ－７．９８；用发光方法与ＤＰＣ放免药盒测定结果的相关系数为０．８３，回归方程为Ｙ＝０６１Ｘ＋６６８。化学发光测定ＣＡ１９－９的试剂成本为２８．２元／个样品，每个样品只需２０ｓ即出具结果，ＣＩＳ放免药盒成本为２８．１元／个样品，６ｈ或更长时间才能出报告，ＤＰＣ为２３元外样品，约２ｈ出结果。结论：化学发光分析虽然成本较贵，但是一种简便、快速的测?
Objective: To introduct the application of chemiluminenscence in clinical sample determination and also compared with radioimmunoassay (RIA). Mathods: The serum sample of CA19 - 9 was obtained from the outclinic and inclinic patients. The equipment of automated chemiluminenseence and the corresponding agent was supplied by Cibe - Chiron company. The RIA kit was supplied by CIS company and DPC company. Dilution recovery, CV% within run and run to run were done. Eighty - one samples were assayed for the concentration of CA 19 - 9 with chemiluminometric technology and CIS RIA kit. Fifty - two of these eighty - one samples were assayed using RIA kit of DPC. The correlation was calculated between the chemiluminenscence assay and radioimmunoassay, and the linear regression analysis betweem the two method were performed. Results: The recovery rate of dilution is 101. 75 - 115. 4 %, the CV% within run and run to run was less 5%. The correlation ratio was 0. 89 between chemiluminenscence and CIS RIA assay, the regression equation was Y - 1. 83 X - 7. 98, the ratio was 0. 83 and the equation was Y = 0. 61 X + 6. 68 between chemiluminenscence and DPC RIA. The reagent cost for each CA19 9 sample was 28. 2 yuan with chemiluminenscence, it cost 28. 1 yuan for CIS RIA assay and 23 yuan for DPC RIA. It took 20 second to give the report, and it took longer for the other two RIA assay. Conclusion: Although it cost much more for hemiluminensence asaay, but it is a simple, quick and no pollution method, the precision is better in our study and worthy of application futher.
出处
《中国临床医学》
2000年第4期390-391,共2页
Chinese Journal of Clinical Medicine
