摘要
目的观察厄洛替尼对人胃癌MKN45细胞的放射增敏作用。方法取对数生长期MKN45细胞,通过MTT法检测厄洛替尼、5-氟尿嘧啶(5-Fu)联合放射线对MKN45细胞的生长抑制作用,计算出厄洛替尼、5-Fu的半数抑制浓度(IC50),比较两者联合放射对MKN45细胞的抑制率;通过集落形成实验检测两者联合放射线对MKN45细胞的增殖抑制作用,应用多靶单击模型计算两者的放射生物学参数:平均致死剂量(D0)、准阈剂量(Dq),比较厄洛替尼、5-Fu的放射增敏比。结果厄洛替尼、5-Fu及放射线均能抑制MKN45细胞的生长,且随着浓度或剂量的增加及作用时间的延长,抑制作用明显增强(P<0.05),厄洛替尼、5-Fu联合放射线对MKN45细胞的抑制作用强于单纯照射(P<0.01);厄洛替尼联合放射线对MKN45细胞的增殖抑制作用明显优于5-Fu联合放射线,两者比较差异有统计学意义(P<0.05),厄洛替尼的放射增敏比为1.54±0.12,明显高于5-Fu的1.24±0.93,两者比较差异有统计学意义(P<0.05)。结论厄洛替尼对MKN45细胞的放射敏感性好于5-Fu,实验结果可为临床提供参考。
Objective To observe the radiation - sensitizing effects of erlotinib on human gastric carcinoma MKN45 cells. Methods Logarithmic growth phase MKN45 cells are used for testing. The growth inhibition on MKN45 cells treaded by erlotinib plus radiation or 5 -fluorouracil （5 -Fu） plus radiation was assessed, and the 50% inhibition concentration （ IC50 ） was calculated for comparison. The proliferation inhibition was assessed by single - cell colony for- mation test. The radiation - sensitizing rates were analyzed by multi - target click model, which provided mean lethal dose, Do and quasi - threshold Dq value. Results Both Erlotinib and radiation could significantly inhibit MKN45 cell growth in concentration - , dose - and duration - dependent manners （ P 〈 0. 05 ）. The inhibition efficacy of erlotinib combined with radiation or 5 - Fu combined with radiation on MKN45 cells was significantly better than radiation alone. The rate of single - cell colony formation of erlotinib plus radiation was significantly higher than 5 - Fu plus radiation （P 〈 0. 05 ）. The radiation - sensitizing rate of erlotinib was significantly higher than that of 5 - Fu （ 1.54 ±0. 12 vs 1.24±0. 93, P 〈 0. 05 ）. Conclusion The radio sensitivity of MKN45 cell treated by erlotinib is better than 5 - Fu.
出处
《广东医学》
CAS
CSCD
北大核心
2013年第21期3238-3242,共5页
Guangdong Medical Journal
基金
河北省承德市科学技术研究与发展计划(自筹经费)项目(编号:201121132)
