摘要
目的通过体外实验探讨人胰腺癌细胞株的放射敏感性。方法培养人胰腺癌细胞株SW1990、Capan1、AsPC1、MIAPaCa2、PANC1、P3,应用集落形成实验测定胰腺癌细胞在6MVX线不同剂量照射后的存活分数,计算各株细胞的放射生物学参数。结果胰腺癌细胞株MIAPaCa2对放射治疗最敏感,SF2为0388,而Capan1对放射治疗最不敏感,SF2为0758,MIAPaCa2与AsPC1、AsPC1与SW1990之间的放射敏感性无显著差异,其余各株胰腺癌细胞之间存在显著差异。结论不同胰腺癌细胞株的放射敏感性存在差异。
Objective To evaluate the radiosensitivity of 6 human pancreatic cancer cell lines in vitro. Methods Human pancreatic cancer cell lines SW1990, Capan-1, AsPC-1, MIAPaCa-2, PANC-1 and P3 were cultured. Their surviving fractions after irradiation using 6MV X-ray at the dose of 0-10 Gy were determined by clonogenic assay. Then their radiosensitivity parameters were calculated. Results The cell lines differed widely in their radiosensitivity (SF2=0.388-0.758). MIAPaCa-2 was most sensitive to X-ray while Capan-1 was most insensitive to it. There was no significant difference in radiosensitivity between MIAPaCa-2 and AsPC-1 as well as between AsPC-1 and SW1999. However, there was marked difference in the radiosensitivity between 2 cell lines of other groups. Conclusions Different cell lines have different radiosensitivities. Thus different radiotherapies should be employed in different patients with pancreatic cancer in clinical practice.
出处
《中华肝胆外科杂志》
CAS
CSCD
2004年第12期821-823,共3页
Chinese Journal of Hepatobiliary Surgery
