摘要
建立了一种以 β 吲哚乙酸为内标物 ,用高效液相色谱内标法直接分离和测定植物中游离糖的新方法。木糖、果糖、葡萄糖、蔗糖、麦芽糖、乳糖和棉子糖的分离在 18min内完成 ;检出限分别达到 1 8μg ,2 3μg ,2 7μg ,1 8μg ,3 5 μg ,4 1μg和 4 3μg ,线性范围为 5 μg/L～ 75 0 μg/L。研究了流动相中乙腈的浓度、pH值对分离 7种糖和 β 吲哚乙酸的影响。方法用于枣和苹果样品的测定并进行回收率试验 ,结果为木糖、果糖、葡萄糖、蔗糖的 5次测定的回收率分别为 97 4%～ 10 2 1% ,97 3%～ 10 1 8% ,98 7%～ 10 2 2 % ,97 7%～ 10 2 5 % ,RSD分别为 1 6 6 %～ 3 45 % ,1 5 4%～ 3 0 1% ,1 2 9%～ 2 91% ,1 0 9%～ 2 6 5 %。
A new approach by which free plant monosaccharides were separated directly and determined with high performance liquid chromatography by using internal standard method was established and β indolylacetic acid was selected as the internal standard substance The separation time for xylose(Xyl), fructose(Fru), glucose(Glu),sucrose(Suc),maltose(Mal),lactose(Lac) and raffinose(Raf) was within 18 min;and the detection limits were 1 8 μg,2 3 μg,2 7 μg,1 8 μg,3 5 μg,4 1 μg and 4 3 μg respectively while the linear dynamics varied within the range of 5 0 μg/L 750 μg/L In this work, the effects of the concentration of CH 3CN and pH values in the mobile phase to the separation of the 7 sugars and β indolylacetic acid were studied as well The method can be used to the determinations of jujube,apple samples Moreover,the recovery experiment was also conducted The results showed that the relative standard deviations of 5 determinations to Xyl,Fru,Glu and Suc were 1 66% 3 45%,1 54% 3 01%,1 29% 2 91%,1 09% 2 65% and the recoveries were 97 4% 102 1%,97 3% 101 8%,98 7% 102 2%,97 7% 102 5%,respectively
出处
《色谱》
CAS
CSCD
北大核心
2000年第6期556-558,共3页
Chinese Journal of Chromatography
关键词
高效液相色谱法
内标法
单糖
植物
测定
high performance liquid chromatography
internal standard method
monosaccharide
β indolylacetic acid
plant
