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类转录激活因子效应物核酸酶(TALENs)的构建及其在基因组定点修饰中的应用 被引量：5

The Construction of Transcription Activator-like Effector Nucleases(TALENs) and Its Application in the Genome Fixed-point Modification
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摘要 类转录激活因子效应物核酸酶(transcription activator-like effector nucleases,TALENs)是一种新发现的基因组定点修饰工具。TALENs由TALE蛋白及II型核酸内切酶Fok I组成,其中TALE由多个重复的氨基酸序列构成,对DNA序列的识别达到一个重复单元结合一个碱基的程度,它在结合Fok I内切酶后就形成了具有DNA定点修饰功能的TALENs。该文主要介绍TALENs的构建及其在基因组定点修饰中的应用。 Transcription activator effector nucleases （TALENs） is a novel DNA editing technology which can induce precise site-specific modification in genome. TALENs consist of two distinct domains, an engineered DNA-binding domain derived from the transcription activator-like effector （TALE） that provides the capability and specificity for binding target DNA sequence, and a non-specific restriction endonuclease Fok I domain fused to the C-terminal of TALE that confers the nuclease activity of TALENs. Here, we summarize the technological back- ground and provide an overview of development and applications of TALENs in genome engineering.
作者 周金伟 王灵慧 申义君 余树民 曹随忠
机构地区 四川农业大学动物医学院 四川大学生命科学学院生物资源与生态环境教育部重点实验室
出处 《中国细胞生物学学报》 CAS CSCD 北大核心 2013年第11期1672-1680,共9页 Chinese Journal of Cell Biology
基金 国家自然科学基金(批准号:31172379)资助的课题~~
关键词 类转录激活因子效应物(TALE) TALENs 基因敲除 基因组定点修饰 TALENs构建 TALE TALENs gene knockout targeted genome modification TALENs construction
分类号 Q78 [生物学—分子生物学]
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共引文献133

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中国细胞生物学学报
2013年 第11期

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