摘要
为探讨我国恶性肿瘤高发区居民饮食中的优势污染霉菌毒素 -脱氧雪腐镰刀菌烯醇 (DON)对人体免疫机能的影响 ,分别以流式细胞光度术 (FCM)、细胞计数、甲基噻唑基四唑 (MTT)比色、双抗体夹心EL ISA方法对 DON作用后人外周血单核细胞 (HPBM)增殖和肿瘤坏死因子 -α(TNF-α)分泌情况进行了研究。FCM检测结果表明 ,HPBM经 PHA预刺激 48h后 ,低浓度 DON(5 0～ 5 0 0 ng/ ml)处理 6 h的细胞增殖指数 (PI)较对照组明显降低 (19.80 %～ 2 6 .0 1%∶ 37.84% ) ;而高浓度 DON (10 0 0～ 2 0 0 0 ng/ ml)处理 2 4h的细胞 PI值则显著高于对照组 (35 .74%～ 34.37%∶ 2 2 .85 % )。细胞计数和 MTT比色法结果显示 ,在 PHA存在的情况下 ,DON可抑制 HPBM的增殖。双抗体夹心 EL ISA法检测结果显示 ,5 0 0和 10 0 0 ng/ ml的 DON可明显降低 HPBM TNF- α分泌 (P<0 .0 1)。研究结果表明 ,根据作用时间和作用浓度不同及有无 PHA刺激 ,DON对 HPBM增殖的影响有所不同 ,但主要表现为对细胞增殖的抑制作用 ,并抑制 TNF- α的分泌 。
Deoxynivalenol (DON) is one of the most common contaminating mycotoxins in food at high risk areas of esophageal carcinoma in China. The effects of DON on proliferation and tumor necrosis factor α (TNF α) secretion of human peripheral blood mononuclear (HPBM) cells in vitro were determined with flow cytometric (FCM) analysis, cell counting, MTT bioassay and ELISA method respectively. FCM analysis revealed that after HPBM were prestimulated with PHA for 48h, the proliferation indexes(PI) of the cells treated with low concentration of DON (50～500ng/ml) for 6h were all dramatically lower than that of normal control (19 80%～26 01% vs 37 84%), while the PI of cells treated with high concentration of DON(1000-2000ng/ml) for 24h were higher than that of normal control (35 74%～34 37%vs 22 85%). Cell number counting and MTT bioassay results showed that at the presence of PHA, DON inhibited the proliferation of HPBM. Double antibody sandwich enzyme linked immunosorbent assay(ELISA) showed that the secretion of TNF α in cells treated with DON(500 1000ng/ml) was significantly lower than that in the control ( P <0 01). The results suggested that DON could either inhibit or stimulate cell proliferation depending on active time, concentration and PHA stimulation. In general, DON mainly inhibited the proliferation of HPBM and decrease TNF α secretion in vitro. Thus, DON might have some negative impacts on human immunological system.
出处
《卫生研究》
CSCD
北大核心
2000年第6期387-389,共3页
Journal of Hygiene Research
基金
河北省自然科学基金资助项目!(No.9735 2 )
