摘要
本实验观察了脱氧雪腐镰刀菌烯醇（ＤＯＮ）对鸡胚关节软骨的作用。结果表明，ＤＯＮ对关节软骨细胞具有明显的毒性作用，其超微结构的改变主要为软骨细胞膜系统的损伤。软骨细胞质膜节段性缺损；线粒体嵴断裂溶解，膜结构模糊；粗面内质网断裂，核蛋白体脱粒；并出现大量变性空泡；软骨基质变疏松、崩解。在给予相同剂量的同时，补充５μｇ硒，软骨细胞损伤程度明显减轻，质膜、核膜均完整，软骨基质未见异常，仅见部分核蛋白体脱粒。本实验证实ＤＯＮ引起骨细胞损伤及硒具有保护作用，可以初步认为在大骨节病的发病上，镰刀菌毒素具有不可忽视的作用。
Ultrastructural observations were carried out on the changes in articular chondrocytes of chicken embryo induced by deoxynivalenol (DON). DON had significant toxic effects on articular chondrocytes. There were obvious lesions observed in the membrane system,including fragmental cell membrane, swollen mitochondria with dristae loss and vacuoluzation, disordered rough endoplasmic reticula with different degree of dilation and fragmentation, accompanied by numerous solutary polyribosomes in the matrix. The local breakage of the nuclear membrane, degenerative vacuoles and myelin figure formation in the cytoplasm were also observed. Selenium dignificantly reduced the ultrastructural lesions of articular chondrocytes infuced by DON. These results suggested that Fusarium mycotoxins and Selenium may play a role in the pathogenesis of Kaschin Beck disease.
出处
《中国地方病学杂志》
CSCD
1994年第2期80-82,121,共4页
Chinese Jouranl of Endemiology
关键词
镰刀菌毒素
硒
软骨细胞
大骨节病
Fusarium mycotoxins
deoxynivalenol
Selenium
articular chondrocyte
Kashin- Beck disease
