摘要
目的采用高效液相色谱法测定芪白平肺胶囊中人参皂苷Rg1与Rb1的含量，为其质量控制提供依据。方法采用WelchMaterialIncC18色谱柱（4．6mm×250mm，5μm）。其流动相：水-乙腈；梯度洗脱（0～10min，81：19；11～35min，81：19—71：29；36～50min，71：29—50：50）；流速：1．2ml／min；检测波长：203nm；柱温：30℃。结果人参皂苷Rg1、Rb1在0．16～2．40μg范围内与其峰面积呈线性关系，r=0．9999（n=8）；人参皂苷Rg1、Rb1的平均加样回收率分别为102．31％、98．55%（n=9）；芪白平肺胶囊中人参皂苷Rg1、Rb1的3批样品测定结果，人参皂苷Rg1平均含量分别为0．332、0．326、0．336mg／g，人参皂苷Rb1平均含量分别为0．496、0．509、0．511mg／g。结论所建立的高效液相色谱方法简便、灵敏度高、重现性好，可用于芪白平肺胶囊中人参皂苷的含量测定。
Objective To establish a method for simultaneous determination of ginsenosides Rg1 and Rb1 in Qibai Pingfei Capsules by high-performance liquid chromatography （HPLC） and to provide a basis for the quality control of Qibai Pingfei Capsules. Methods HPLC was performed on a Welch Material Inc C18 column （4.6 mm × 250 mm, 5 μm） with a mobile phase of wateacetonitrile for gradient elution at a flow rate of 1..2 ml/min, a detection wavelength of 203 nm, and a column temperature of 30 ℃. The water/acetonitrile ratio was 81：19 at 0-10 min, ranged from 81：19 to 71：29 at 11-35 min, and ranged from 71：29 to 50：50 at 36-50 min. Results Ginsenosides Rg, and Rb1 showed a linear relationship between concentration and peak area within 0.16-2.40 μg （r= 0. 999 9, n=8）; the average recovery rates of ginsenosides Rg1 and Rb1 were 102.31% and 98.55%, respectively （n=9）. In the three batches of Qibai Pingfei Capsule samples, the average contents of ginsenoside Rg1 were 0. 332, 0. 326, and 0. 336 mg/g, respectively, and the average contents of ginsenoside Rb1 were 0. 496, 0. 509, and 0. 511 mg/g, respectively. Conclusion This HPLC method is simple, with high sensitivity and good repeatability. It can be used for the determination of ginsenosides ig1 and Rb1 in Qibai Pingfei Capsules.
出处
《安徽中医学院学报》
CAS
2013年第5期72-75,共4页
Journal of Anhui Traditional Chinese Medical College
基金
安徽省高校省级自然科学研究项目(KJ2011Z218)
安徽省2012年中医发展省级专项(皖中医药[2012]10号)
关键词
高效液相色谱法
梯度洗脱
芪白平肺胶囊
人参皂苷
含量测定
high-performance liquid chromatography
gradient elution
Qibai Pingfei Capsule
Ginsen- oside
determination
