摘要
目的:应用超高速液相色谱(RRLC)法、常规高效液相色谱(HPLC)法和MerckC18整体柱HPLC法测定红参、西洋参中人参皂苷Rg1、Re和Rb1含量的分析比较。方法:分别采用Agilent Zorbax Eclipse XDB-C18(50mm×4.6mm,1.8μm)色谱柱、DiamonsilC18(200mm×4.6mm,5μm)色谱柱和MerckC18(100mm×4.6mm,2μm)整体柱,以乙腈-水(红参)和乙腈-0.1%磷酸溶液(西洋参)为流动相,梯度洗脱,流速1.0mL.min-1,检测波长203nm,以外标法进行定量。结果:RRLC法和MerckC18整体柱的应用与常规C18柱的HPLC法相比,对红参、西洋参中人参皂苷Rg1、Re和Rb1的含量测定结果差异不大,但可显著缩短分析检验时间。结论:RRLC法和MerckC18整体柱HPLC法简便快捷,分离度好,结果准确,重复性好。
Objective：To compare a rapid resolution liquid chromatography（RRLC） and two HPLC methods for determining the compounds ginsenoside Rg1,Re and Rb1in Radix et Rhizoma Ginseng Rubra and Radix Panacis Quinuefolii.Methods：The separation was performed on Agilent Zorbax Eclipse XDB-C18（50 mm×4.6 mm,1.8 μm）column,Diamonsil C18（200 mm×4.6 mm,5 μm） column and Merck monolithic C18 column（100 mm×4.6 mm,2 μm） by gradient elution.The mobile phase consisted of acetonitrile-water for Radix et Rhizoma Ginseng Rubra or acetonitrile-0.1% phosphoric acid for Radix Panacis Quinuefolii.The flow rate was 1.0 mL·min-1 with detection wavelength 203 nm.Results：The contents of Rg1,Re and Rb1 determined by the developed methods showed no differences,but the analysis time was greatly shortened.Conclusion：The developed RRLC and HPLC with Merck C18 column methods are rapid,accurate and reproducible for quality control of Radix et Rhizoma Ginseng Rubra and Radix Panacis Quinuefolii.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第9期1720-1724,共5页
Chinese Journal of Pharmaceutical Analysis
