摘要
本文介绍一种高分子聚合物HFMC注入输精管,遇体液即合成有孔隙(孔径2—16u)的固态聚合物,附着管壁并缓慢释放适度的H^+,以改变精子存活环境,从而达到猕猴非阻断性的输精管避孕。在猕猴配对试验中证明,输精管注入HFMC 30mg×2(n=2),在注射后47—50天精液品质即逐渐恢复,已有致孕能力;输精管注入HFMC 60mg×2(n=4),在300天后,有些个体精液晶质才逐渐恢复,注后348—353天之间已有50％的雄猴配对致孕;另有50％的雄猴在注入854天时精液品质仍处于不育水平。实验结果还表明:HFMC对精子具有致死,致畸率高及使精子活力显著下降,这种作用随HFMC剂量的增大而加强,这种影响也随时间的后移而逐渐减弱;HFMC对输精管具有可通性及可复性。今后如能在注入剂量,自溶稳定性方面再改进,HFMC有可能成为男性节育较为理想的一种新型避孕剂。
The results of injection of a high molecular copolymer HFMC into vas deferens of male rhesus monkey is preliminarily reported. While HFMC mixed with body fluid in the vas deferens, it immediately polymerized into solid polymer with porosity and attached to the wall of the vas deferens immediately, and released certain amount of H+ gradually, thus the living environment of sperms was changed.
In the matching experiment of the rhesus monkey, We could find the difference in fertility resumption, with various dosages of HFMC. In the 30 mg HFMC X 2 intra vas deferens group (n = 2), the rate of recovered fertility was 100% within 2-5 months, but in 60 mg HFMC x 2 intra vas deferens group (n = 4 ) that the rate was only 50% within 2 years of period.
Experimental results demonstrated that injection of HFMC induced lethat effects and caused abnormality of sperms and thus obviously decreased sperm vitality. Such effects became stronger and stronger as the dosage of HFMC increased, such influence gradually weakened and disappeared as the length of time after injection of HFMC incrased, The degree of activity of sperms in the semen deterimination were determined by routine methods of WHO that the results analysed was consistent with the results of matching experiment.
We are planning to continue the experiment by improvement on the stability of self solubility and the dosage of injection. It is quite possible that HFMC may be a new type of contraceptive for man.
基金
国家计划生育委员会
四川省计划生育委员会资助课题
关键词
猕猴
输精管
注射
HFMC
生育
HFMC (Hydrogel for male contraception), Contraceptive of vas deferens, Oligasthenoteratozoospermia
