摘要
本文用9种限制性内切酶(BamH I,BgIⅠ,BgIⅡ,EcoRⅠ,EcoR Ⅴ,PstⅠ,PvuⅡ,SalⅠ,XhoⅠ)分析大熊猫的线粒休DNA(mtDNA)。构建其中5种酶(BamHⅠ,EcoRⅠ,EcoRⅤ,PstⅠ,PvuⅡ)的mtDNA物理图谱。大熊猫mtDNA的分子大小约为16.4 Kb,酶切位点是随机分布。我们的结果为进一步研究大熊猫mtDNA进化提供了基础资料。
Mitochondrial DNA from giant panda has been digested with nine enzymes: BamH I , Bgl I , Bgl II , EcoR I , EcoR V, Pst I , Pvu II , Sal I and Xho I , and the molecular weights of the fragments have been determinde. BamH I , EcoR I , EcoRV and Xho I cleave giant panda mtDNA at three pointes yielding three fragments, respectively. Digestion with Pst I and Pvu II produces two fragments, rerpectiuely. Cleavage with Bgl I , Bgl II and Sal I generates one fragment, respectively. Based on analysis of a set of fragments generated by single and double-digestions with these restriction endonucleases, BamH I , Pst I , EcoR V and Pvu II cleavage sites were located on the map with EcoR I sites as reference points. The molecular size of mtDNA from giant panda is about 16.4 Kb. the distribution of cleavage sites on mtDNA is not random. Our preliminary results may be useful for the evolutionary study of mtDNA from giant panda.
基金
中国科学院重大项目--野生动物细胞库的建立及应用课题资助
