摘要
以十一种限制性内切酶对鲢鱼ｍｔＤＮＡ进行单酶完全酶解的切点数：ＳａｌＩ和ＢｇｌⅡ为０；ＰｓｔＩ．ＸｈｏＩ．ＫｐｎＩ和ＳａｃＩ为１；ＨｐａＩ和ＸｂａＩ为２；ＢａｍＨＩ为３；ＢｇｌＩ和ＥｃｏＲＩ为４。以九种限制性内切酶对鳙鱼ｍｔＤＮＡ进行单酶完全酶解，其切点数；ＰｓｔＩ．ＸｈｏＩ．ＳａｌＩ．ＫｐｎＩ．ＢｇｌⅡ均为１；ＨＰａＩ和ＸｂａＩ为２；ＳａｃＩ为３；ＢｇｌＩ为４。采用琼脂糖凝胶电泳法测得鲢鱼ｍｔＤＮＡ分子量为１５９３０±２２０碱基对（ｂｐ）；鳙鱼ｍｔＤＮＡ分子量为１６６５０±１５０碱基对（ｂｐ）。用单、双酶解法和部份酶解法构建了鲢鱼九种酶１８个切点和鳙鱼九种酶１６个切点的限制性内切酶酶切图谱。另外，对这两种鱼的酶解位点数，片段大小和限制酶图谱进行了比较。
The mitochondrial DNA (mtDNA) of silver carp were digested by 11 restriction endonucleases which were Burn I,Bgl I,Bgl Ⅱ,EcoR I,Hpa I,Kpn I,Pst I,Sac I,Sal I,Xba Iand XhoI. The fragments cleaved by these enzymes are 3,4,0,4, 2, 1,1, 1, 0, 2 and 1, respectively. The mtDNA of bighead were digested by restriction endonucleases Bgl I, Bgl Ⅱ, Hpa I,RpnI,PstI,Sac I,Sal I, Xba I and Xho I into 4,1,2,1,1,3, 1,2 and 1 fragments, respectively. The sizes of mtDNA were estimated by agarose gel electrophoresis and they are 15930 ±220 bp for silver carp and 16650 ±150bp for bighead. The maps of 9 restriction endonucleases for silver carp (18 cleaved sites) and bighead (16 cleaved sites) were constructed after digesting by the single,double or partial enzyme. The paper also compared the enzyme-digested sites,the size of fragment and the maps of restriction endonucleased between both fishes.
出处
《水产学报》
CAS
CSCD
北大核心
1994年第3期221-230,共10页
Journal of Fisheries of China
基金
国家自然科学基金
关键词
鲢鱼
鳙鱼
限制性内切酶
DNA
silver carp,bighead,mtDNA restriction physical map
