摘要
本试验利用ＡｐａⅠ、ＢａｍＨⅠ、ＢｃｌⅠ、ＢｇｌⅠ、ＢｇｌⅡ、ＣｌａⅠ、ＤｒａⅠ、ＥｃｏＲⅠ、ＥｃｏＲⅤ、ＨａｅⅡ、ＨｉｎｄⅢ、ＫｐｎⅠ、ＰｓｔⅠ、ＰｖｕⅡ、ＳａｃⅠ、ＳａｌⅠ、ＳｍａⅠ和ＸｈｏⅠ计１８种限制性内切酶，研究了来自欧洲、非洲及国内的５个山羊品种共计３３只个体的ｍｔＤＮＡ，共检测出２７种限制性态型，可归结为８种单倍型。结果表明，国内２个山羊品种的基本单倍型为ＢａｍＨⅠ－Ａ、ＢｃｌⅠ－Ｂ、ＣｌａⅠ－Ａ、ＥｃｏＲⅠ－Ａ、ＥｃｏＲⅤ－Ａ、ＨａｅⅡ－Ａ、ＨｉｎｄⅢ－Ｃ和ＰｖｕⅡ－Ａ，以及为ＢａｍＨⅠ－Ａ、ＢｃｌⅠ－Ａ、ＣｌａⅠ－Ａ、ＥｃｏＲⅠ－Ａ、ＥｃｏＲⅤ－Ａ、ＨａｅⅡ－Ａ、ＨｉｎｄⅢ－Ｃ和ＰｖｕⅡ－Ａ，而欧洲及非洲３个山羊品种基本的单倍型为ＢａｍＨⅠ－Ｂ、ＢｃｌⅠ－Ａ、ＣｌａⅠ－Ａ、ＥｃｏＲⅠ－Ａ、ＥｃｏＲⅤ－Ａ、ＨａｅⅡ－Ａ、ＨｉｎｄⅢ－Ｂ和ＰｖｕⅡ－Ａ，这一结果提示本研究几个受试山羊品种可能有两种不同的母系来源；同时，各品种的分子聚类图也表明国内２个山羊品种间具有较近的亲缘关系，而欧洲品种和非洲３个品种间的亲缘关系较近，这一结果也支持上述几个受试山羊品种可能有不同野生祖先的结论，同时也验证了欧洲?
Eighteen restriction endonucleases, ApaⅠ, BamHⅠ, BclⅠ, BglⅠ, BglⅡ, ClaⅠ, DraⅠ, EcoRⅠ, EcoRⅤ, HaeⅡ, HindⅢ, KpnⅠ, PstⅠ, PvuⅡ, SacⅠ, SalⅠ, SmaⅠand XhoⅠwere used to investigate the mitochondrial DNA polymorphism of different goat breeds from Europe, Africa and China Among the 33 individuals of 5 goat breeds analyzed in this paper, 27 restriction morphs were detected which could be sorted into 8 haplotypes The results indicated that the main haplotype of internal goat breeds was Ⅰ and Ⅲ, but Ⅳ for goat breeds of Europe and Africa It was pointed out that the goat breeds analyzed might originate from two different maternal side Meanwhile, the molecular dendrogram of different populations showed that the internal goat breeds had the close relationship, so did the European and African goat breeds It was also verified that the Aferican goat breed, Nubian goat was once introduced into the European goat breed, Sanne goat
基金
国家科委基础司专项经费
国家杰出青年基金
云南省科委"二百人计划"资助项目
