摘要
椒样薄荷幼苗在含有0.5%(w/v)NaCl的Hoagland营养液中生长72 h后,不定根的伸长量约为对照组一半。分别向含有0.5%(w/v)NaCl的营养液中,加入不同浓度(50、100、200、300μmol/L)的SNP,用于培养椒样薄荷幼苗,72 h后观测根系生长状况。当SNP浓度为100μmol/L时,不定根的伸长量、根毛密度、根毛总长度与未添加SNP的对照组相比均有明显增加。通过组织染色观察到,NO能够缓解NaCl对根尖顶端分生组织的脂质过氧化作用及对质膜完整性的损伤,并正向调节内源ROS水平。
Elongation of the adventitious root was about a half of no NaCI adding control group after the growth of peppermint seedlings in 0.5％ （w/v） NaCI contained Hoagland nutrient solution for 72 h.Sodium nitroprusside （SNP） of different concentrations （50,100,200,300 μmol/L） was added in 0.5％ （w/v） NaCl contained Hoagland nutrient solutions to cultivate peppermint seedlings.Growth situation of adventitious roots was determined 72 hours later.Elongation of adventitious root,total length of root hair and root hair density are significantly increased than those of peppermint cultivated in no SNP solutions when SNP concentration is 100 μmol/L.Histochemical staining reveals that SNP treatment can alleviate peroxidation of membrane lipid and damage to plasma membrane integrity.SNP treatment can also positively regulate intracellular reactive oxygen species （ROS） level.
出处
《山东科学》
CAS
2013年第5期33-38,共6页
Shandong Science
基金
科技部国际合作项目(2011DFA30990
2007DFA30630)
