摘要
为探讨盐胁迫对从甘肃不同地区植物根际分离出的木霉菌生长的影响,筛选出耐盐性较强的木霉菌,利用不同浓度NaCl溶液胁迫下,基于生长速率和产孢量,筛选和测定耐盐性较强的木霉菌。结果表明,从采自张掖和武威等地6份植物根际土样中分离得到的8株木霉菌中,木霉CT-3和木霉YT-3表现出较好的耐盐性,在浓度为60mg·mL^(-1)NaCl溶液胁迫下培养6 d后,木霉CT-3和木霉YT-3分离株的生长速率与无盐胁迫对照相比无显著差异(P>0.05)。相同条件下2株木霉分离株产孢量均高于其他木霉分离株,分别为1.95×10^(7)和2.31×10^(7)cfu·mL^(-1),但低于无盐胁迫对照。因此,盐浓度≤60 mg·mL^(-1)时,木霉CT-3和木霉YT-3分离株具有较强的耐盐性,对于发展微生物肥料具有良好的应用前景。
The aim of this study was to investigate the effects of salt stress on the growth of Trichoderma isolates collected from the rhizospheres of plants in different regions and identify salt-tolerant Trichoderma isolates.Salt-tolerant Trichoderma isolates were screened under different NaCl solutions by determining colony growth rates and the number of spores produced.Our results showed that isolates Trichoderma CT-3 and Trichoderma YT-3 had the highest levels of salt tolerance among the eight Trichoderma isolates collected from six rhizosphere soil samples from Zhangye and Minqin.Trichoderma CT-3 and Trichoderma YT-3 isolates exhibited high salt tolerance and presented no significant difference in colony growth compared with the control condition(0 mg·mL^(-1)NaCl)after incubation for 6 days at an NaCl concentration of 60 mg·mL^(-1)(P>0.05).Spore production was higher in Trichoderma CT-3(1.95×10^(7)cfu·mL^(-1))and Trichoderma YT-3(2.31×10^(7)cfu·mL^(-1))than in the other six isolates under the same conditions,but lower than spore production under the control conditions.Therefore,Trichoderma CT-3 and Trichoderma YT-3 exhibit high salt tolerance at concentrations equal to or lower than 60 mg·mL^(-1).Therefore,they have good prospects for application in microbial fertilizer.
作者
景涛
李俊义
李昕冉
马耀杰
郭彩苹
孙晨曦
徐秉良
张树武
JING Tao;LI Junyi;LI Xinran;MA Yaojie;GUO Caiping;SUN Chenxi;XU Bingliang;ZHANG Shuwu(College of Plant Protection,Gansu Agricultural University/Engineering Laboratory for Biological Control of Crop Diseases and Pests,Lanzhou 730070,Gansu,China)
出处
《草业科学》
CAS
CSCD
北大核心
2023年第3期665-673,共9页
Pratacultural Science
基金
甘肃农业大学“伏羲杰出人才培育计划”项目(Gaufx-03J03)
甘肃农业大学人才专项经费(2017RCZX-07)
国家自然基金(31860526)
甘肃省杰出青年基金(18JR3RA161)。
关键词
盐胁迫
根际土壤
分离
木霉
耐盐性
抑制率
产孢量
筛选
salt stress
rhizosphere soil
isolation
Trichoderma
salt tolerance
inhibitory rates
spore production
screening
