摘要
目的:探讨有氧运动训练对小鼠心脏形态结构、功能及心室肌微小RNAs(micro RNAs,miRNAs)的影响,研究关健miRNA在心脏对运动适应过程中的调节作用。方法:KM雄性小鼠随机分为安静对照组和8周有氧运动训练组,每组各10只。有氧运动训练组小鼠进行为期8周的无负重有氧训练,每周训练5天,前5周,每天早上固定时间运动1次,后3周每天在早上和晚上固定时间分别运动1次;第1周每次运动时间为30min,随后每周增加10min直至90min。有氧运动训练完成后,首先使用超声心动图检测全部小鼠心脏各项形态学和功能学指标,随后将小鼠颈椎脱位猝死取心肌组织,使用基因芯片扫描两组差异表达的miRNAs,使用实时定量PCR技术验证miRNA-1,-133a,-29和-30c4个关键差异表达的miRNA,同时使用荧光定量PCR和酶联免疫吸附技术检测小鼠心肌组织结缔组织生长因子(connective tissue growth factor,CTGF)基因和蛋白的表达。结果:有氧运动训练明显提高了小鼠心室内径、心壁厚度和功能学指标Ratio E/A,提示运动诱导小鼠心脏生理性肥大,增加了心室顺应性;与安静对照组相比,8周有氧运动训练后小鼠心肌组织中miRNA-1和-133a显著下降(P<0.05),而miRNA-29和-30c则显著升高(P<0.05);同时有氧运动训练组小鼠心肌组织中CTGF基因和蛋白的表达低于对照组,差异具有统计学意义(P<0.05);进一步研究发现,有氧运动训练组小鼠心肌组织中miRNA-30c表达量与CTGF蛋白表达量呈负相关(相关系数r=-0.818,P=0.004)。结论:有氧运动训练通过增加心室肌组织中miRNA-30c的表达量,抑制心肌组织中CTGF的表达和浓度,从而提高运动引起心脏生理性肥大过程中心室顺应性,有利于心脏功能的正常有效发挥。
Objective：To explore the effects of aerobic exercise training on the cardiac structure and miRNAs, and analyze their role in cardiac adaption induced by aerobic exercise training. Methods： Male KM mice （n= 10/group） were randomized into two groups： 8 weeks aerobic exercise training group and sedentary control group. Mice in training group undertook a swim- ming protocol for 8 weeks, 5days/wk, from the 1st to 5th week, mice were trained one time a day in the morning, from the 6th to 8th week, mice were trained twice a day with a 12 h inter- val. In the 1st week, the training time was 30 min, and the time was increased 10 min per week until to 90 min. After the aerobic exercise training, all mice were measured by echocar- diography. MiRNAs analysis was performed by miRNA microarray and confirmed by real-time PCR. The level of connective tissue growth factor （CTGF） gene expression was determined by real-time PCR, CTGF protein concentration was assessed by ELISA. Results：Aerobic exercise training improved aerobic capacity, left ventricular and induced cardiac hypertrophy. MiRNAs- 1 and 133 a were siginificant downregulated in training group （ P 〈 0.05 ）, however, miRNAs- 29c and 30c expression increased in training group （P〈0.05）, correlated with a decrease in CTGF gene expression in training group （P〈0.05）. Furthermore, miRNA-30 c was inversely correlated to CTGF protein contration in training group （r =-0.818, P= 0.05）. Conclusion： Aerobic exercise training could increase miRNA-30c expression and decrease CTGF gene ex- pression and concentration in the heart, which is relevant to the improved left ventricular com- pliance and beneficial cardiac effects.
出处
《体育科学》
CSSCI
北大核心
2013年第9期70-76,共7页
China Sport Science
关键词
有氧运动
心脏
心室顺应性
微小RNA
鼠
动物实验
aerobic exercise
heart
ventricular compliance
miRNA
mice
animal experiment
