摘要
目的:建立颈动脉缩窄术心衰小鼠模型,观察长期游泳运动对心衰小鼠左心室miRNA-350及其靶基因JNK表达的影响,以探讨有氧运动改善心脏疾病的可能机制。方法:雄性C57BL/6小鼠分为对照组(C组,n=8只)、心衰模型组(HFM组,n=8只)和心衰模型运动组(HFME组,n=8只)。超声心动图仪检测各组小鼠心脏形态和功能参数;分别用qRT-PCR和Western Blotting技术检测各组小鼠左心室miRNA-350、JNK mRNA和JNK蛋白的表达水平。结果:TAC手术后28 d时,HFM组和HFME组PWTS、PWTD、LVEDD、IVSTD和IVSTS显著高于C组,EF显著低于C组。取材前1周,HFM组和HFME组PWTS、PWTD、LVEDD、IVSTD和IVSTS等显著高于C组,EF显著低于C组;HFME组LVEDD和EF显著高于HFM组。末次训练后,HFM组左心室miRNA-350表达水平显著高于C组,HFME组左心室miRNA-350表达水平显著低于HFM组,但明显高于C组;HFME组左心室JNK mRNA表达水平显著高于C组和HFM组;HFME组左心室JNK蛋白表达水平虽明显高于HMF组,但显著低于C组。结论:TCC手术成功复制了心衰小鼠模型,表现为PWTS、PWTD、LVEDD、IVSTD和IVSTS显著增厚和EF显著降低。运动改善小鼠心衰表征的机制可能与运动抑制miRNA-350的表达,进而增强JNK mRNA和JNK蛋白的表达有关。
Objective： This study established heart failure model of mice by carotid artery coaretation and observed the effects of long-term swimming on expressions of miRNA - 350 and JNK gene, in order to explore the mechanism of im proving heart disease by aerobics. Methods： Twenty-four male C57BL/6 rats were divided into control group （ C, n = 8）, heart failure model group （HFM, n =8） and heart failure model combined exercise/swimming （HFME, n =8） The morphological and functional parameters of heart were measured by eehocardiogram. The expressions of miRNA - 350, JNK mRNA and JNK protein were determined by qRT-PCR and western blotting, Results： PWTS, PWTD, LVEDD, IVSTD, IVSTS in HFM group and HFME group are higher than in C group, and EF is lower than in C group, 28 days after carotid artery coarctation and one week before last day. Also LVEDD and EF in HFME group are higher than in HFM group one week before last day. Left ventricle＇ s rniRNA - 350 expression in HFM group is higher than in HFME and C groups after training, and it in HFME group is higher than in C group. JNK mRNA expression of left ventricle in HFME group is higher than in C and HFM groups. JNK protein expression of left ventricle in HFME group is lower than in I-IFM group, but higher than in C group. Conclusion： Carotid artery eoaretation established heart failure model of mice, which characterized by increases of PWIX3, PWTD, LVEDD, IVSTD and decline of EF. The mechanism of the fact that exercise can imorove heart disease is that exercise can inhibit the expression of miRNA -350 and en-hance mRNA and protein expressions of JNK.
出处
《北京体育大学学报》
CSSCI
北大核心
2014年第5期77-83,共7页
Journal of Beijing Sport University
基金
南通大学航海医学研究所"特种医学"学科招标课题(HYS-2013-004)
