摘要
目的 从血型、染色体核型、DNA可变数串联重复序列 (VNTR)几个方面进行对比研究以更好地评估异基因移植后的植入状态及其与疾病复发的关系。方法 采用聚合酶链反应 (PCR)方法扩增D17S30、D1S80、ApoB3个不同位点的多态性 ,对 15例白血病患者异基因外周血造血干细胞移植 (allo PBSCT)后的嵌合状态进行检测 ,对其中 7例异性间移植和 3例血型不合移植又分别进行了染色体核型分析和全套血型分析。结果 经VNTR检测 ,allo PBSCT后 15天有 11例呈供体型嵌合状态 ,4例呈混合型嵌合状态。 7例异性间移植者经染色体核型分析证实为完全嵌合状态。 3例ABO血型不一致者在移植后出现混合血型 ,最早 1例移植后 2个月转变为供体的血型 ,有 1例移植后 7个月持续呈混合状态。结论 PCR扩增VNTR片段评估allo PBSCT后的嵌合状态具有方法简便、灵敏度高的优点 ,特别适合供受体间性别、血型、HLA配型完全相同的异基因造血干细胞移植。
Objective To evaluate engraftment status of patients after allogeneic peripheral blood stem cell transplantation (allo PBSCT) based on red blood cell type, karyotype and variable number of tandem repeats (VNTR). Methods Amplification of 3 loci of VNTR in vitro by polymerase chain reaction (PCR) was preformed to detect the polymorphism of 15 patients post PBSCT. Furthermore, analysis of karyotype in 7 sex mismatched patients and red blood cell antigen switching in 3 ABO blood type mismatched patients were also employed. Results VNTR analysis in 15 patients revealed complete chimerism in 11 patients and mixed chimerism in 4. In the 7 sex mismatched patients, karyotype analysis showed types of their donors. As for blood type analysis, it was after 2 months that the first patient transformed to his donor's type. One patient showed mixed status 7 months after transplantation. Conclusion Amplification of VNTR loci by PCR for documenting engraftment status after allo PBSCT is simple and sensitive. This technology is especially suitable for patients who have received stem cells with the same sex, same blood type and identical HLA type. Examination with several different VNTR loci will improve the power of discrimination.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2000年第11期823-825,共3页
National Medical Journal of China
基金
国家自然科学基金!资助项目 (39870 710 )
上海市卫生系统"百人计划"资助
