摘要
目的探讨异基因脐血干细胞移植植活证据检测中简单易行的方法。方法取供者和受者移植前及移植后15、300d的标本,抽提DNA。根据已发表的基因序列设计合成两对引物,采用PCR-SSP方法检测MN血型基因型。结果供者为NN型,受者移植前为MM型,移植后15 d为MN型(嵌合型),移植后300 d为NN型(供者型)。NN基因在移植植活证据中作为供者的标记物在受者体内发现。结论采用MN血型基因型检测作为植活证据,可以对异基因脐血干细胞移植进行早期监控,且标本量小,较其他方法更加敏感。
Objective To establish a simple and practical method to assess the outcome of allogeneic transplantation of hematopoietic stem cells from umbilical cord blood. Methods The DNA was extracted from the samples collected from the donor and the receptor separately before and 15 and 300 d after transplantation. MN genotype was determined by PCR with sequence-specific primers (PCR-SSP) with the 2 pairs of specific primers designed and synthesized on the basis of reported MN gene sequence. Results The donor was identified as having NN genotype while the recipient shown to be MM genotype. MN geneotype was detected in the recipient (mixed chimerism) at 15 d after transplantation. The genotype reversed to NN (donor origin) at 300 d after transplantation. The NN gene, as the donor's marker and evidence for the graft survival analysis, was eventually identified in the recipient. Conclusions With MN genotype test to confirm the graft survival, early engraftment monitor of allogeneic umbilical cord blood transplantation is ensured, with the advantages of better sensitivity and requiring small amount of sample (0.2 ml). It may also facilitate subsequent therapeutic decisions.
出处
《第一军医大学学报》
CSCD
北大核心
2002年第10期912-914,共3页
Journal of First Military Medical University
基金
安徽省自然科学基金(00057142)
合肥市重点攻关项目(2000-E-01)
关键词
基因型
脐血干细胞移植
异基因
移植物存活
genotype
umbilical stem cell transplantation
allogeneic
graft survival
