摘要
为评估异基因外周血造血干细胞移植 (ALLO PBSCT)后的植入状况 ,采用细胞遗传学分析结合短串联重复序列 聚合酶链反应 (STR PCR) ,对 3 0例Allo PBSCT病人进行了 1～ 2 5个月的随访观察。结果表明 ,不同性别移植的 2 3例 ,2 1例完全嵌合 (CC) ,1例混合嵌合 (MC) ,1例为完全受者核型 ;相同性别移植的慢性髓性白血病 (CML) 7例 ,除 2例外 ,其余均未检测到Ph染色体 ;STR PCR检测结果与染色体分析结果完全一致。说明两种方法具有互补性 。
To evaluate engraftment state after allogeneic peripheral blood stem cell transplantation (ALLO PBSCT), 30 patients who received ALLO PBSCT were studied using cytogenetic analysis and short tandem repeats polymerase chain reaction (STR PCR). Twenty one of the 23 patients who received sex mismatched donor marrows showed complete chimerism (CC), one showed mixed chimerism (MC), and one showed completely recipient karyotype. In the 7 patients of chronic myelogenous leukemia (CML) who received sex matched donor marrows, except 2 patients, the rest showed completely disappearance of Ph positive cells. The results from these two detection methods completely coincided mutually. STR PCR and cytogenetic analysis can be supplementary to each other and their results can be pooled together.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2002年第12期1102-1104,共3页
Medical Journal of Chinese People's Liberation Army
