摘要
目的：建立蜂胶毛胶中总黄酮以及高良姜素、白杨素含量测定方法，测定不同基原毛胶中上述各指标的含量。方法：以芦丁为对照品，采用uV法测定总黄酮含量，检测波长为415nm；用HPLC同时测定高良姜素、白杨素含量，采用Welchrom C18柱，以甲醇-0．15％磷酸溶液梯度洗脱，检测波长为268nm。结果：总黄酮在0～1．248mg线性关系良好，r=0．9999（n=6），平均回收率为98．82％，RSD=1．45％。高良姜素、白杨素分别在0．089～0．4450，0．115～0．580μg线性关系良好，r均达到1．000，平均回收率分别为99．03％、99．32％，RSD分别为1．10％、1．72％。结论：该方法准确、可靠、简便易行，可用于蜂胶毛胶的定量分析，为蜂胶毛胶、其提取物以及蜂胶制品的质量评价和控制提供了依据。
Objective： To establish UV and HPLC methods for determination of total flavonoids, galangin and chrysin in Propolis. Methods： UV method was used to determine total flavonoids, and the detection wavelength was 415 nm. The analysis of Galangin and Chrysin was performed on a Welchrom CIS column with a gradient solvent system of methanol-0. 15% phosphoric acid solution, and the detection wavelength was 268 nm, Results： The linear range of rutin was 0 - 1. 248 mg（r =0. 999 9） ,and the average recovery was 98.82% with RSD 1.45%. The linear range of galangin and ehrysin in propolis were 0. 089 -0. 445 0μg and 0. 115 - 0. 580 μg, both with good correlation（ r = 1. 000）. The average recovery were 99. 03% for galangin and 99.32% for ehrysin,and RSD were 1.10% and 1.72% , respectively （ n = 6）. Conclusion： The methods are simple and reliable fo and products, and can be used for quality control and evaluation.
出处
《中国现代中药》
CAS
2013年第8期655-658,共4页
Modern Chinese Medicine
基金
浙江省分析测试科技计划项目(2011C37066)
关键词
蜂胶毛胶
总黄酮
高良姜素
白杨素
基原
含量分析
Propolis
Total flavonoids
Galangin
Chrysin
Origin r quantitative analysis of propolis, its extracts
Content determination
