摘要
目的:建立测定蜂胶中白杨素和高良姜素的反相高效液相色谱法,并用该方法测定9个产地蜂胶样品中白杨素和高良姜素的含量。方法:采用Zorbax SB-C_(18)(150mm×4.6mm,5μm)色谱柱;流动相:甲醇-0.1%甲酸;梯度洗脱:0→35min,甲醇-0.1%甲酸比例由(50∶50)→(75∶25);流速:1.0mL·min^(-1);检测波长:267nm;柱温:30℃。结果:白杨素在0.08～4.1μg(r=0.9999)范围内线性关系良好,其高、中、低3个量的平均加样回收率分别为99.1%,101.2%,99.7%;RSD分别为1.3%,1.8%,1.4%。高良姜素在0.06～3.8μg(r=0.9998)范围内线性关系良好,其高、中、低3个量的平均加样回收率分别为99.7%,100.6%,100.2%;RSD分别为1.0%,1.7%,1.8%。9个蜂胶样品中白杨素和高良姜素含量范围分别为2.25%～5.58%和2.18%～3.44%。结论:该方法是一种灵敏、准确的分析方法。
Objective：To develop an HPLC method for determination of chrysin and galangin in propolis. Method： RP- HPLC was used. The separation was performed on Zorbax SB -Clscolumn （150 mm×4.6mm,5μm） at a temperature of 30 ℃. The mobile phase consisted of methanol （A） and 0. 1% formic acid （B） （v/v） using a line- ar gradient elution of 50% A→75% A at 0→35 min. The flow rate was 1.0 mL · min^-1. The detection was clone at 267 nm. Results： The linear range for chrysin and galangin was 0. 08 - 4. 1 μg （ r = 0. 9999） and 0. 06 - 3.8 μg （ r =0. 9998 ）, respectively. In the high, medium and low concentration, the average recoveries of chrysin were 99. 1% ,101.2% and 99. 7% with corresponding RSD of 1.3% ,1.8%and 1.4% ;the average recoveries of galangin were 99. 7%, 100. 6% and 100. 2% with corresponding RSD of 1.0%, 1.7% and 1.8%. Conclusion： The method is sensitive and accurate for the determination of chrysin and galangin in propolis.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第4期522-524,共3页
Chinese Journal of Pharmaceutical Analysis
基金
陕西省科技攻关项目(2004k03-G8)
陕西省博士后科研经费项目(BX03003)
西北大学2004年度重点科研基地开放基金(2004-29)
